The ribosome inhibitor chloramphenicol induces motility deficits in human spermatozoa: A proteomic approach identifies potentially involved proteins
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Le résumé fourni par la source
capacitation. But the type of ribosomes involved (cytoplasmic or mitochondrial) is still debated. Here, we investigate the presence and activity of the two types of ribosomes in mature human spermatozoa. By targeting ribosomal RNAs and proteins, we show that both types of ribosomes are localized in the midpiece as well as in the neck and the base of the head of the spermatozoa. We assessed the impact of cycloheximide (CHX) and chloramphenicol (CP), inhibitors of cytoplasmic and mitochondrial ribosomes, respectively, on different sperm parameters. Neither CHX, nor CP impacted sperm vitality, mitochondrial activity (measured through the ATP content), or capacitation (measured through the content in phosphotyrosines). However, increasing CP concentrations induced a decrease in total and progressive motilities as well as on some kinematic parameters while no effect was observed with CHX. A quantitative proteomic analysis was performed by mass spectrometry in SWATH mode to compare the proteomes of spermatozoa capacitated in the absence or presence of the two ribosome inhibitors. Among the ∼700 proteins identified in the different tested conditions, 3, 3 and 25 proteins presented a modified abundance in the presence of 1 and 2 mg/ml of CHX, and 1 mg/ml of CP, respectively. The observed abundance variations of some CP-down regulated proteins were validated using Multiple-Reaction Monitoring (MRM). Taken together, our results are in favor of an activity of mitochondrial ribosomes. Their inhibition by CP results in a decrease in the abundance of several proteins, at least FUNDC2 and QRICH2, and consequently induces sperm motility deficits.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- The ribosome inhibitor chloramphenicol induces motility deficits in human spermatozoa: A proteomic approach identifies potentially involved proteins
- Date Crossref
- 02/09/2022
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Mons Laboratory of Cell Biology pays non établi dans la noticeUniversité ou école supérieure
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Birmingham Women’s and Children’s NHS Foundation Trust pays non établi dans la noticeÉtablissement de santé
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University of Birmingham Centre for Systems Modelling and Quantitative Biomedicine pays non établi dans la noticeUniversité ou école supérieure
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Université Libre de Bruxelles Evolutionary Biology and Ecology pays non établi dans la noticeUniversité ou école supérieure
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CHU Ambroise Paré pays non établi dans la noticeÉtablissement de santé
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National Health Service pays non établi dans la noticeÉtablissement de santé
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Clinique de Fertilité Régionale de Mons pays non établi dans la noticeÉtablissement de santé
Laboratory of Cell Biology — University of Mons, Birmingham Women’s and Children’s NHS Foundation Trust et Centre for Systems Modelling and Quantitative Biomedicine — University of Birmingham, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.