AMPK/mTOR down-regulated autophagy enhances aberrant endometrial decidualization in folate deficient pregnant mice

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Supplementary Methods: 1 Mouse endometrial stromal cell isolation and culture; 2. Immunofluorescence cytology. 2. Supplementary Figures: Figure S1. The serum folate concentration was detected by electro-chemiluminescence immunoassay; Figure S2. Cell indentification was induced by hormone treatment in primary mESCs (in vitro); Figure S3. The key mRNA expressions of autophagy by qRT-PCR in primary mESCs (in vitro). Supplementary Tables: Table S1. Antibodies were used in western blot; Table S2. Sequences of forward and reverse primers were used in RT-PCR (5’→3’).

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