Single-cell transcriptomes identifies characteristic features of mouse macrophages in liver Mallory-Denk bodies formation
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Le résumé fourni par la source
Mallory-Denk bodies (MDBs) consist of intracellular aggregates of misfolded proteins in ballooned hepatocytes and serve as important markers of progression in certain liver diseases. Resident hepatic macrophage-mediated inflammation influences the development of chronic liver diseases and cancer. Here, the first systematic study of macrophages heterogeneity in mice was conducted to illustrate the pathogenesis of MDB formation using single-nucleus RNA sequencing (snRNA-seq). Furthermore, we provided transcriptional profiles of macrophages obtained from the fractionation of mouse liver tissues following chronic injury. We equally identified seven discrete macrophage subpopulations, each involved in specific cellular activated pathways such as basal metabolism, immune regulation, angiogenesis, and cell cycle regulation. Among these, a specific macrophage cluster (Cluster4), a subpopulation specifically expressing genes that regulate cell division and the cell cycle, was identified. Interestingly, we found that CCR2 was significantly induced in Cluster2, thereby inducing monocytes to migrate to macrophages to promote MDB pathogenesis. Thus, our study is the first to demonstrate the heterogeneity of macrophages associated with liver MDB formation in mice through single-cell resolution. This serves as the basis for further insights into the pathogenesis of liver MDB formation and molecular mechanisms of chronic liver disease progression.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Single-cell transcriptomes identifies characteristic features of mouse macrophages in liver Mallory-Denk bodies formation
- Date Crossref
- 01/08/2022
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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First Affiliated Hospital of Guangzhou Medical University pays non établi dans la noticeÉtablissement de santé
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First Affiliated Hospital of Guangdong Pharmaceutical University Clinical Laboratory pays non établi dans la noticeÉtablissement de santé
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Guangzhou Medical University The State Key Laboratory of Respiratory Disease pays non établi dans la noticeUniversité ou école supérieure
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Second Affiliated Hospital of Guangzhou Medical University pays non établi dans la noticeÉtablissement de santé
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State Key Laboratory of Respiratory Disease pays non établi dans la noticeStructure de recherche
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School of Basic Medical Sciences pays non établi dans la noticeUniversité ou école supérieure
First Affiliated Hospital of Guangzhou Medical University, Clinical Laboratory — First Affiliated Hospital of Guangdong Pharmaceutical University et The State Key Laboratory of Respiratory Disease — Guangzhou Medical University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.