Platelet transfusions in a murine model of neonatal polymicrobial sepsis: Divergent effects on inflammation and mortality
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Le résumé fourni par la source
BACKGROUND: Platelet transfusions (PTxs) are often given to septic preterm neonates at high platelet count thresholds in an attempt to reduce bleeding risk. However, the largest randomized controlled trial (RCT) of neonatal transfusion thresholds found higher mortality and/or major bleeding in infants transfused at higher thresholds. Using a murine model, we investigated the effects of adult PTx on neonatal sepsis-induced mortality, systemic inflammation, and platelet consumption. STUDY DESIGN AND METHODS: Polymicrobial sepsis was induced via intraperitoneal injection of cecal slurry preparations (CS1, 2, 3) into P10 pups. Two hours after infection, pups were transfused with washed adult Green Flourescent Protein (GFP+) platelets or control. Weights, platelet counts, and GFP% were measured before 4 and 24 h post-infection. At 24 h, blood was collected for quantification of plasma cytokines. RESULTS: The CS batches varied in 24 h mortality (11%, 73%, and 30% in CS1, 2, and 3, respectively), due to differences in bacterial composition. PTx had differential effects on sepsis-induced mortality and systemic inflammatory cytokines, increasing both in mice infected with CS1 (low mortality) and decreasing both in mice infected with CS2 and 3. In a mathematical model of platelet kinetics, the consumption of transfused adult platelets was higher than that of endogenous neonatal platelets, regardless of CS batch. DISCUSSION: Our findings support the hypothesis that transfused adult platelets are consumed faster than endogenous neonatal platelets in sepsis and demonstrate that PTx can enhance or attenuate neonatal inflammation and mortality in a model of murine polymicrobial sepsis, depending on the composition of the inoculum and/or the severity of sepsis.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Platelet transfusions in a murine model of neonatal polymicrobial sepsis: Divergent effects on inflammation and mortality
- Date Crossref
- 06/05/2022
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Boston Children's Hospital Division of Newborn Medicine pays non établi dans la noticeÉtablissement de santé
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Harvard University pays non établi dans la noticeUniversité ou école supérieure
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University at Buffalo pays non établi dans la noticeUniversité ou école supérieure
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Boston Children's Museum pays non établi dans la noticeInstitution
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Mount Sinai Beth Israel pays non établi dans la noticeÉtablissement de santé
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Beth Israel Deaconess Medical Center pays non établi dans la noticeÉtablissement de santé
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Beth Israel Deaconess Hospital pays non établi dans la noticeÉtablissement de santé
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Brigham and Women's Hospital Division of Transfusion Medicine pays non établi dans la noticeÉtablissement de santé
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Department of Pediatrics Harvard Medical School Boston Massachusetts USA pays non établi dans la noticeInstitution
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Department of Pharmaceutical Sciences University of Buffalo Department of Pharmaceutical Sciences pays non établi dans la noticeUniversité ou école supérieure
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Institutional Centers for Clinical and Translational Research pays non établi dans la noticeStructure de recherche
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Division of Neonatology Beth Israel Medical Center Boston Massachusetts USA Division of Neonatology pays non établi dans la noticeÉtablissement de santé
Division of Newborn Medicine — Boston Children's Hospital, Harvard University et University at Buffalo, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.