Anticancer Activity of Cordia dichotoma against a Panel of Human Cancer Cell Lines and Their Phytochemical Profiling via HPLC and GCMS
Rattachement africain : in, sa, bd. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
(bark, leaves, pulp and seed). The plant material was collected from UT of J&K and methodical bioassays were carried out on ten human cancer cell lines (Michigan Cancer Foundation-7 (MCF-7), M.D. Anderson-Metastatic Breast (MDA-MB-231), Neuroblastoma-2a (N2A), SH-SY5Y, U-251, HCT-116, SW-620, A-549, MIA PaCa-2, Panc-1) from five different origins (breast, CNS, colon, lung, pancreas) respectively. Methanolic extracts were produced and fractions were then obtained from the extracts and evaluated for cytotoxicity. Mechanistic assays, HPLC, and GCMS profiling were performed on the highest active fraction. The Sulforhodamine B (SRB) assay determined the in vitro cytotoxicity. The findings revealed that the bark portion had in vitro cytotoxicity against the A-549 human lung cancer cell line. To our knowledge, this is the first study to show that the plant's bark has anticancer properties and induced chromatin condensation, confirmed cell death via ROS generation, and significantly decreased colony formation in A-549 cell line from lung origin in a dose-dependent manner. Furthermore, HPLC and GCMS investigations indicated the presence of a number of bioactive molecules such as gallic acid (144,969.86) uV*sec, caffeic acid (104.26) uV*sec, ferulic acid (472.87) uV*sec, vanillic acid (13,775.39) uV*sec, palmitic acid (18.34%), cis vaccenic acid (28.81%), etc. and one of the compounds was reported for the first time from the bark. As a result of its promising efficacy, it may become an essential cancer chemopreventive or chemotherapeutic medication for patients with lung carcinoma.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Anticancer Activity of Cordia dichotoma against a Panel of Human Cancer Cell Lines and Their Phytochemical Profiling via HPLC and GCMS
- Date Crossref
- 28/03/2022
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu pays non établi dans la noticeUniversité ou école supérieure
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Indian Institute of Integrative Medicine Cancer Pharmacology Division pays non établi dans la noticeStructure de recherche
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King Abdulaziz University Princess Dr Najla Bint Saud Al-Saud Center for Excellence Research in Biotechnology pays non établi dans la noticeUniversité ou école supérieure
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King Saud bin Abdulaziz University for Health Sciences pays non établi dans la noticeUniversité ou école supérieure
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Daffodil International University Department of Public Health pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Basic Sciences Division of Biochemistry pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Science Department of Biological Sciences pays non établi dans la noticeUniversité ou école supérieure
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Cancer Pharmacology Division — Indian Institute of Integrative Medicine et Princess Dr Najla Bint Saud Al-Saud Center for Excellence Research in Biotechnology — King Abdulaziz University, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.