Cytotoxic and other bioactivities of a novel and known sesquiterpene lactones isolated from Vernonia leopoldi (Sch. Bip. ex Walp.) Vatke in breast cancer cell lines
Rattachement africain : se, Éthiopie. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
(Sch. Bip. ex Walp.) Vatke (Asteraceae) is one of the widely used anti-cancer traditional medicinal plants in Ethiopia, despite the lack of data to support its therapeutic efficacy. Here we describe the isolation of compounds from the plant and the investigation of their cytotoxicity and other bioactivities. We identified the novel sesquiterpene lactone (SL) 11ß,13-dihydrovernodalol along with the three other SLs (vernomenin, vernolepin, and 11ß,13-dihydrovernodalin) and three flavonoids (apigenin, eriodyctiol, and luteolin) isolated from this plant for the first time. The structures of all the compounds were established based on extensive analysis of nuclear magnetic resonance spectroscopic data and confirmed by high-resolution electrospray ionization mass spectrometry. We then studied the biological activities of the SLs and found that all were cytotoxic at low μM ranges against MCF-7 and JIMT-1 breast cancer cells as well as against the normal-like MCF-10A breast epithelial cells evaluated in a spectrophotometric assay. All the SLs significantly reduced JIMT-1 cell migration after 72 h of treatment with 2 μM concentrations in a wound healing assay. Treatment with all SLs reduced the aldehyde dehydrogenase expressing cancer stem cell sub-population of the JIMT-1 cells significantly, evaluated by flow cytometry. Only 11ß,13-dihydrovernodalin resulted in a significant inhibition of tumor necrosis factor-α-induced translocation of nuclear factor κB to the cell nucleus. In addition, we show that the reporter fluorophore nitrobenzoxadiazole (NBD) can successfully be conjugated with an SL and that this SL-NBD conjugate is taken up efficiently in JIMT-1 cells. Therefore, the overall bioactivities of the SL compounds and specifically their effects against the stemness of breast cancer cells make them prime candidates for further in-depth investigation.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Cytotoxic and other bioactivities of a novel and known sesquiterpene lactones isolated from Vernonia leopoldi (Sch. Bip. ex Walp.) Vatke in breast cancer cell lines
- Date Crossref
- 01/01/2022
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Lund University pays non établi dans la noticeUniversité ou école supérieure
-
Hawassa University Hawassa University, Éthiopie (code pays fourni par la source)Université ou école supérieure
-
Addis Ababa University Addis Ababa University, Éthiopie (code pays fourni par la source)Université ou école supérieure
-
Armauer Hansen Research Institute Addis Ababa, Éthiopie (code pays fourni par la source)Structure de recherche
-
Faculty of Natural Science Department of Biology pays non établi dans la noticeUniversité ou école supérieure
-
Hawassa College of Teacher Education Department of Biology Hawassa College of Teacher Education, Éthiopie (pays nommé en fin d’affiliation)Université ou école supérieure
-
College of Natural and Computational Sciences Department of Microbial Addis Ababa University, Éthiopie (pays nommé en fin d’affiliation)Université ou école supérieure
-
Faculty of Science Centre for Analysis and Synthesis pays non établi dans la noticeUniversité ou école supérieure
-
School of Medicine Department of Biochemistry Addis Ababa University, Éthiopie (pays nommé en fin d’affiliation)Université ou école supérieure
Lund University, Hawassa University (Hawassa University, Éthiopie) et Addis Ababa University (Addis Ababa University, Éthiopie), avec 6 autres affiliations. Pays d’affiliation : Éthiopie.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.