A 3D Bioprinted Human Meniscus Shape Enriched with Mesenchymal Cells
Rattachement africain : ro. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Background and objectives: Regenerative medicine, with its massive development over the years, has the potential to solve some of the most problematic medical issues, such as functional organ transplantation. The aim of this study was to create a human meniscal shape 3D-printed enriched with human adipose-derived mesenchymal cells. Materials and Methods: Human infrapatellar fat pad was harvested, and mesenchymal cells were isolated. The mesenchymal stem cells were differentiated to the chondrocite lineage and a hydrogel (a nanofibrillar cellulose, sodium alginate, D-mannitol, and Hepes buffer solution combination) cell mixture was bioprinted to create three human-size meniscus structures. The obtained structures were evaluated regarding the cell viability, appropriate size in relation to a native meniscus, and some mechanical characteristics. Results: The human meniscal shape created respected the anatomic characteristic of a native structure. Cell viability of approximately 97% and extracellular matrix formation after the printing process were observed. The mean maximum force for the meniscus with mesenchymal cells was 6.5 N (+/−0.5 N) compared to the mean maximum force for the native meniscus of 10.32 N (+/−0.7 N), which is statistically relevant (p < 0.01). Conclusion: This paper presents the potential of bioprinting viable cell structures that could in the future present enough mechanical strength to replace a human organ, such as a meniscus. There are still limitations regarding the ink and the printing process, but we are confident that these problems will soon be solvable.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A 3D Bioprinted Human Meniscus Shape Enriched with Mesenchymal Cells
- Date Crossref
- 10/12/2021
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Victor Babeș University of Medicine and Pharmacy Timișoara pays non établi dans la noticeUniversité ou école supérieure
-
Spitalul Clinic Judeţean de Urgenţă "Pius Brînzeu" Timişoara pays non établi dans la noticeÉtablissement de santé
-
Polytechnic University of Timişoara pays non établi dans la noticeUniversité ou école supérieure
-
“Victor Babes” University of Medicine and Pharmacy Timisoara Department of Orthopaedics pays non établi dans la noticeUniversité ou école supérieure
-
“Victor Popescu” Emergency Military Hospital Timisoara pays non établi dans la noticeÉtablissement de santé
-
"Victor Popescu" Emergency Military Hospital Timisoara pays non établi dans la noticeÉtablissement de santé
-
"Victor Babes" University of Medicine and Pharmacy Timisoara Department of Orthopaedics pays non établi dans la noticeUniversité ou école supérieure
-
Pius Brinzeu Emergency Clinical County Hospital Timisoara pays non établi dans la noticeÉtablissement de santé
-
University Politehnica Timișoara Department of Mechanics and Strength of Materials pays non établi dans la noticeUniversité ou école supérieure
Victor Babeș University of Medicine and Pharmacy Timișoara, Spitalul Clinic Judeţean de Urgenţă "Pius Brînzeu" Timişoara et Polytechnic University of Timişoara, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.