The Formation of Melanocyte Apoptotic Bodies in Vitiligo and the Relocation of Vitiligo Autoantigens under Oxidative Stress
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Le résumé fourni par la source
Background. Oxidative stress has a vital role in the early stages of vitiligo. Autoantigens released from apoptotic melanocytes (MC) under oxidative stress are involved in the presentation and recognition of antigens. However, the transport of autoantigens to the cell surface and their release to the extracellular environment are still unclear. Apoptotic bodies (ABs) have always been considered as a key source of immunomodulators and autoantigens. Yet, the role of ABs in the immune mechanism of vitiligo is still unknown. Purpose. To explore whether MC’s autoantigens translocate into ABs during oxidative stress‐induced apoptosis and study the molecular mechanisms underlying autoantigen migration and AB formation. Methods. PIG3V (an immortalized human vitiligo melanocyte cell line) were treated with H2O2, and ABs were separated. Transmission electron microscopy, flow cytometry, Western blot, mass spectrometry, and other methods were used to determine the relocation of specific antigens in PIG3V cells to ABs. After pretreatment with specific inhibitors (Rho kinase (Y‐27632), myosin light chain kinase (MLCK, ML‐9), pan‐caspase (zVAD‐FMK), and JNK (SP600125)), the pathway of autoantigen translocation into ABs and the formation of apoptotic bodies were determined. Results. When treated with 0.8 mM H2O2, ABs were released from these cells. Autoantigens such as tyrosinase‐related protein 1 (TYRP‐1) and cleavage nuclear membrane antigen Lamin A/C (Asp230) were concentrated in ABs. The expression of autoantigens and the formation of ABs increased in a time‐ and dose‐dependent manner after treatment with H2O2, while the application of specific inhibitors inhibited the formation of apoptotic bodies, i.e., the expression of antigens. Conclusion. Vitiligo autoantigens translocate into ABs in the process of apoptosis induced by oxidative stress. The cytoskeletal protein activation pathway and the JNK‐related apoptosis pathway are involved in the transport of autoantigens and the formation of ABs. ABs may be the key bridge between MC cell apoptosis and cellular immunity.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- The Formation of Melanocyte Apoptotic Bodies in Vitiligo and the Relocation of Vitiligo Autoantigens under Oxidative Stress
- Date Crossref
- 01/01/2021
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Shanxi Medical University pays non établi dans la noticeUniversité ou école supérieure
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Shaanxi Provincial People's Hospital Department of Dermatology pays non établi dans la noticeÉtablissement de santé
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Shanxi Provincial People’s Hospital pays non établi dans la noticeÉtablissement de santé
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Hebei North University pays non établi dans la noticeUniversité ou école supérieure
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Anhui Medical University pays non établi dans la noticeUniversité ou école supérieure
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Air Force Engineering University pays non établi dans la noticeUniversité ou école supérieure
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Air Force General Hospital PLA pays non établi dans la noticeÉtablissement de santé
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Chinese People's Armed Police General Hospital pays non établi dans la noticeÉtablissement de santé
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Xijing Hospital pays non établi dans la noticeÉtablissement de santé
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Air Force Medical University pays non établi dans la noticeUniversité ou école supérieure
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Air Force Medical Center Department of Dermatology pays non établi dans la noticeÉtablissement de santé
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Armed Police Forces Beijing General Hospital Department of Dermatology pays non établi dans la noticeÉtablissement de santé
Shanxi Medical University, Department of Dermatology — Shaanxi Provincial People's Hospital et Shanxi Provincial People’s Hospital, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.