The H2S-Donor Erucin Exhibits Protective Effects against Vascular Inflammation in Human Endothelial and Smooth Muscle Cells
Rattachement africain : it. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Preservation of vascular wall integrity against degenerative processes associated with ageing, fat-rich diet and metabolic diseases is a timely therapeutical challenge. The loss of endothelial function and integrity leads to cardiovascular diseases and multiorgan inflammation. The protective effects of the H2S-donor erucin, an isothiocyanate purified by Eruca sativa Mill. seeds, were evaluated on human endothelial and vascular smooth muscle cells. In particular, erucin actions were evaluated on cell viability, ROS, caspase 3/7, inflammatory markers levels and the endothelial hyperpermeability in an inflammatory model associated with high glucose concentrations (25 mM, HG). Erucin significantly prevented the HG-induced decrease in cell viability as well as the increase in ROS, caspase 3/7 activation, and TNF-α and IL-6 levels. Similarly, erucin suppressed COX-2 and NF-κB upregulation associated with HG exposure. Erucin also caused a significant inhibition of p22phox subunit expression in endothelial cells. In addition, erucin significantly prevented the HG-induced increase in endothelial permeability as also confirmed by the quantification of the specific markers VE-Cadherin and ZO-1. In conclusion, our results assess anti-inflammatory and antioxidant effects by erucin in vascular cells undergoing HG-induced inflammation and this protection parallels the preservation of endothelial barrier properties.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- The H2S-Donor Erucin Exhibits Protective Effects against Vascular Inflammation in Human Endothelial and Smooth Muscle Cells
- Date Crossref
- 15/06/2021
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
University of Pisa Department of Pharmacy pays non établi dans la noticeUniversité ou école supérieure
-
Research Centre for Cereal and Industrial Crops pays non établi dans la noticeStructure de recherche
-
Cereal Research Centre pays non établi dans la noticeStructure de recherche
-
University of Siena Department of Life Sciences pays non établi dans la noticeUniversité ou école supérieure
-
University of Basilicata Department of Science pays non établi dans la noticeUniversité ou école supérieure
-
University of Florence Department of Neuroscience pays non établi dans la noticeUniversité ou école supérieure
Department of Pharmacy — University of Pisa, Research Centre for Cereal and Industrial Crops et Cereal Research Centre, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.