A direct role for SNX9 in the biogenesis of filopodia.
Rattachement africain : gb. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Filopodia are finger-like actin-rich protrusions that extend from the cell surface and are important for cell-cell communication and pathogen internalization. The small size and transient nature of filopodia combined with shared usage of actin regulators within cells confounds attempts to identify filopodial proteins. Here, we used phage display phenotypic screening to isolate antibodies that alter the actin morphology of filopodia-like structures (FLS) in vitro. We found that all of the antibodies that cause shorter FLS interact with SNX9, an actin regulator that binds phosphoinositides during endocytosis and at invadopodia. In cells, we discover SNX9 at specialized filopodia in Xenopus development and that SNX9 is an endogenous component of filopodia that are hijacked by Chlamydia entry. We show the use of antibody technology to identify proteins used in filopodia-like structures, and a role for SNX9 in filopodia.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
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Où se fait cette recherche
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The Gurdon Institute pays non établi dans la noticeStructure de recherche
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AstraZeneca (United Kingdom) pays non établi dans la noticeEntreprise
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University of Cambridge Gurdon Institute and Department of Biochemistry pays non établi dans la noticeUniversité ou école supérieure
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Antibody Discovery and Protein Engineering pays non établi dans la noticeInstitution
The Gurdon Institute, AstraZeneca (United Kingdom) et Gurdon Institute and Department of Biochemistry — University of Cambridge, avec 1 autre affiliation.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.