Molecular characteristics and in vitro effects of antimicrobial combinations on planktonic and biofilm forms of Elizabethkingia anophelis
Rattachement africain : tw. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
OBJECTIVES: To investigate the in vitro activity of antibiotics against clinical Elizabethkingia anophelis isolates and to find a suitable antibiotic combination with synergistic effects to combat antibiotic-resistant E. anophelis and its associated biofilm. METHODS: E. anophelis isolates were identified by 16S rRNA sequencing; 30 strains with different pulsotypes were identified and the MIC, antibiotic resistance mechanism, antibiotic combination activity and killing effects of antimicrobial agents on biofilms of these strains were determined. RESULTS: All E. anophelis isolates were susceptible to minocycline and cefoperazone/sulbactam (1:1). More than 90% of clinical isolates were susceptible to cefoperazone/sulbactam (1:0.5), piperacillin/tazobactam and rifampicin. Some novel mutations, such as gyrA G81D, parE D585N and parC P134T, that have never been reported before, were identified. The synergistic effect was most prominent for the combination of minocycline and rifampicin, with 93.3% of their FIC index values ≤0.5, and no antagonism was observed using the chequerboard method. This synergistic effect between minocycline and rifampicin was also observed using time-killing methods for clinical E. anophelis isolates at both normal inoculum and high inoculum. Twenty-nine isolates tested positive for biofilm formation. Minocycline remained active against biofilm-embedded and biofilm-released planktonic E. anophelis cells; however, the enhanced effect of minocycline by adding rifampicin was only observed at 24 h (not at 72 and 120 h). CONCLUSIONS: Although E. anophelis was resistant to many antibiotics and could exhibit biofilm formation, minocycline showed potent in vitro activity against this pathogen and its associated biofilm.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Molecular characteristics and <i>in vitro</i> effects of antimicrobial combinations on planktonic and biofilm forms of <i>Elizabethkingia anophelis</i>
- Date Crossref
- 03/02/2021
- Éditeur
- Oxford University Press (OUP)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Chi Mei Medical Center Department of Medical Research pays non établi dans la noticeÉtablissement de santé
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National Yang Ming Chiao Tung University pays non établi dans la noticeUniversité ou école supérieure
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Taipei Veterans General Hospital Department of Medicine pays non établi dans la noticeÉtablissement de santé
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National Yang Ming University Hospital Institute of Emergency and Critical Care Medicine pays non établi dans la noticeÉtablissement de santé
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National Chiayi University Department of Food Science pays non établi dans la noticeUniversité ou école supérieure
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Chia Nan University of Pharmacy and Science pays non établi dans la noticeUniversité ou école supérieure
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National Cheng Kung University Hospital Department of Internal Medicine pays non établi dans la noticeÉtablissement de santé
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National Cheng Kung University pays non établi dans la noticeUniversité ou école supérieure
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Kaohsiung Veterans General Hospital Department of Internal Medicine pays non établi dans la noticeOrganisme public
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College of Sustainable Environment Department of Occupational Safety and Health/Institute of Industrial Safety and Disaster Prevention pays non établi dans la noticeUniversité ou école supérieure
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College of Medicine Department of Medicine pays non établi dans la noticeUniversité ou école supérieure
Department of Medical Research — Chi Mei Medical Center, National Yang Ming Chiao Tung University et Department of Medicine — Taipei Veterans General Hospital, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.