Long Non-coding RNA SNHG17 Upregulates RFX1 by Sponging miR-3180-3p and Promotes Cellular Function in Hepatocellular Carcinoma
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Le résumé fourni par la source
Background Hepatocellular carcinoma (HCC) is one of the most common types of cancer that is associated with poor quality of life in patients and a global health burden. The mechanisms involved in the development and progression of HCC remain poorly understood. Methods Hepatocellular carcinoma human samples and cell lines were subjected to qRT-PCR for expression assessment. CCK-8 assay, Transwell migration and invasion assay, were applied for cell function detection. Animal experiment was used to measure the function of SNHG17 on cell growth in vivo. Western blot was conducted to evaluate the level of EMT in cells. RIP, RNA pull-down and luciferase reporter assays were performed to assess the correlation between SNHG17, miR-3180-3p and RFX1. Results Our study demonstrated that SNHG17 was upregulated in HCC human samples and involved cell proliferation, migration, invasion progress. SNHG17 promoted HCC cell growth and metastasis in vivo. Furthermore, we investigated the downstream factor of SNHG17, SNHG17 acted as a molecular sponge for miR-3180-3p, and SNHG17 regulated RFX1 expression via miR-3180-3p. SNHG17 promotes tumor-like behavior in HCC cells via miR-3180-3p/RFX1. Conclusion We determined RFX1 as the target of miR-3810-3p; SNHG17 enhanced the progression of HCC via the miR-3180-3p/RFX1 axis. Taken together, our findings may provide insight into the molecular mechanism involved in the progression of HCC and develop SNHG17 as a novel therapeutic target against HCC.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Long Non-coding RNA SNHG17 Upregulates RFX1 by Sponging miR-3180-3p and Promotes Cellular Function in Hepatocellular Carcinoma
- Date Crossref
- 15/01/2021
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Chengdu University of Traditional Chinese Medicine Department of Clinical Laboratory pays non établi dans la noticeUniversité ou école supérieure
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The Eighth Hospital of Xi'an pays non établi dans la noticeÉtablissement de santé
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Affiliated Hospital of Shaanxi University of Chinese Medicine pays non établi dans la noticeÉtablissement de santé
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Shaanxi University of Chinese Medicine Department of General Surgery pays non établi dans la noticeUniversité ou école supérieure
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Chinese Academy of Medical Sciences & Peking Union Medical College pays non établi dans la noticeUniversité ou école supérieure
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Nanjing Medical University National Health Council (NHC) Key Laboratory of Living Donor Liver Transplantation pays non établi dans la noticeUniversité ou école supérieure
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Wuhan Eighth Hospital Department of Gastroenterology pays non établi dans la noticeÉtablissement de santé
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College of Basic Medicine pays non établi dans la noticeUniversité ou école supérieure
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Key Laboratory of Liver Transplantation pays non établi dans la noticeStructure de recherche
Department of Clinical Laboratory — Chengdu University of Traditional Chinese Medicine, The Eighth Hospital of Xi'an et Affiliated Hospital of Shaanxi University of Chinese Medicine, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.