Si‐Wu‐Tang Alleviates Nonalcoholic Fatty Liver Disease via Blocking TLR4‐JNK and Caspase‐8‐GSDMD Signaling Pathways
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Le résumé fourni par la source
BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) has high global prevalence; however, the treatments of NAFLD are limited due to lack of approved drugs. METHODS: Mice were randomly assigned into three groups: Control group, NAFLD group, NAFLD plus Si-Wu-Tang group. A NAFLD mice model was established by feeding with a methionine- and choline-deficient (MCD) diet for four weeks. Si-Wu-Tang was given orally by gastric gavage at the beginning of 3rd week, and it lasted for two weeks. The treatment effects of Si-Wu-Tang were confirmed by examining the change of body weight, serum alanine aminotransferase (ALT) and aspartate transaminase (AST) levels, Oil Red O staining, and hematoxylin and eosin (H&E) staining of the liver samples and accompanied by steatosis grade scores. The expression and activation of the possible signaling proteins involved in the pathogenesis of NAFLD were determined by western blotting. RESULTS: Mice fed with four weeks of MCD diet displayed elevated serum levels of ALT and AST, while there was decreased body weight. The hepatic Oil Red O staining and H&E staining showed severe liver steatosis with high steatosis grade scores. All these can be improved by treating with Si-Wu-Tang for two weeks. Mechanistically, the increased hepatic TLR4 expression and its downstream JNK phosphorylation induced by MCD diet were suppressed by Si-Wu-Tang. Moreover, the upregulations of Caspase-8, gasdermin D (GSDMD), and cleaved-GSDMD in liver mediated by MCD diet were all inhibited by Si-Wu-Tang. CONCLUSIONS: Treatment with Si-Wu-Tang improves MCD diet-induced NAFLD in part via blocking TLR4-JNK and Caspase-8-GSDMD signaling pathways, suggesting that Si-Wu-Tang has potential for clinical application in treating NAFLD.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Si‐Wu‐Tang Alleviates Nonalcoholic Fatty Liver Disease via Blocking TLR4‐JNK and Caspase‐8‐GSDMD Signaling Pathways
- Date Crossref
- 01/01/2020
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Sun Yat-sen University Department of Traditional Chinese Medicine pays non établi dans la noticeUniversité ou école supérieure
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Third Affiliated Hospital of Sun Yat-sen University pays non établi dans la noticeÉtablissement de santé
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Anhui Xinhua University pays non établi dans la noticeUniversité ou école supérieure
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The Seventh Affiliated Hospital of Sun Yat-sen University pays non établi dans la noticeÉtablissement de santé
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Guangdong Pharmaceutical University Department of Science and Education pays non établi dans la noticeUniversité ou école supérieure
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School of Biomedical Engineering pays non établi dans la noticeUniversité ou école supérieure
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Zhongshan School of Medicine pays non établi dans la noticeUniversité ou école supérieure
Department of Traditional Chinese Medicine — Sun Yat-sen University, Third Affiliated Hospital of Sun Yat-sen University et Anhui Xinhua University, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.