Artesunate exerts anti-prolactinoma activity by inhibiting mitochondrial metabolism and inducing apoptosis
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Le résumé fourni par la source
Background: Prolactinoma is the most common hormone-secreting pituitary adenoma. Dopamine receptor agonists (DAs) are effective in reducing prolactin levels and tumor mass, but some prolactinoma patients are resistant to DAs. Treating patients with DA-resistant prolactinoma is challenging. In this study, we examined the anti-prolactinoma effect of artesunate (ART), a potential new treatment option for prolactinoma, and its mechanism of action. Methods: Cell Counting Kit-8 (CCK8) and flow cytometry were used to detect the effect of ART on the proliferation, cycle, and apoptosis of rat pituitary adenoma cell line MMQ. The subcellular localization of ART was observed using confocal fluorescence microscopy. The JC-1 mitochondrial membrane potential (MMP) detection and Seahorse assays were used to detect the effect of ART on mitochondrial function. Real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis were used to detect the effect of ART on the expression of prolactin (PRL) and apoptosis-related proteins. A mouse xenograft model of prolactinoma was used to detect the inhibitory effect of ART on MMQ in vivo. Results: ART specifically inhibited MMQ proliferation and PRL synthesis, induced G0/G1 phase arrest and apoptosis in vitro. ART accumulated in the mitochondria of MMQ cells, inhibiting mitochondrial respiratory function and mediating apoptosis through the mitochondrial pathway. ART also inhibited proliferation and activated the apoptosis of MMQ cells in vivo. Conclusions: ART has a strong inhibitory effect on prolactinoma both in vitro and in vivo, and its effects rely on high MMP to inhibit mitochondrial metabolism and induce apoptosis. Our results provide evidence for ART as a candidate drug for the treatment of prolactinoma.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Artesunate exerts anti-prolactinoma activity by inhibiting mitochondrial metabolism and inducing apoptosis
- Date Crossref
- 01/07/2020
- Éditeur
- AME Publishing Company
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Sun Yat-sen University Department of Neurosurgery pays non établi dans la noticeUniversité ou école supérieure
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Fifth Affiliated Hospital of Sun Yat-sen University pays non établi dans la noticeÉtablissement de santé
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Yangzhou University Department of Neurosurgery pays non établi dans la noticeUniversité ou école supérieure
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Third Affiliated Hospital of Guangzhou Medical University Department of Pathology pays non établi dans la noticeÉtablissement de santé
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Guangzhou Medical University pays non établi dans la noticeUniversité ou école supérieure
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Guangdong Pharmaceutical University Department of Histology and Embryology pays non établi dans la noticeUniversité ou école supérieure
Department of Neurosurgery — Sun Yat-sen University, Fifth Affiliated Hospital of Sun Yat-sen University et Department of Neurosurgery — Yangzhou University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.