Immune responses in beta-thalassaemia: heme oxygenase 1 reduces cytokine production and bactericidal activity of human leucocytes
Rattachement africain : th, jp, gb. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Patients with beta-thalassaemia increase the risk of bacterial infections, particularly Burkholderia pseudomallei (Bp), the causative agent of melioidosis in Thailand. Impaired immune cell functions may be the cause of this susceptibility, but detailed mechanisms have not been defined. In this study, we observed impaired production of IFN-gamma and IL-10 by whole blood from beta-thalassaemia patients upon stimulation with a range of bacteria-derived stimuli. In contrast, IFN-gamma response via TCR and plasma IgG specific for Bp were still intact. Importantly, mRNA expression of heme oxygenase 1 (HO-1), a potential modulator of immune function, was increased in whole blood from beta-thalassaemia patients, either with or without stimulation with Bp in vitro. Induction of HO-1 by hemin or CoPP in vitro reduced production of IFN-gamma and IL-10 from healthy human PBMCs and decreased bacterial clearance activity of whole blood from healthy controls and beta-thalassaemia, while inhibition of HO-1 by SnPP enhanced both functions in healthy controls. These results were confirmed to some extent in purified human monocytes of healthy controls. Our results suggest a mechanism that excess hemin of beta-thalassaemia patients is a significant cause of immune suppression via HO-1 induction and may underlie the susceptibility of these individuals to severe bacterial infection.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Immune responses in beta-thalassaemia: heme oxygenase 1 reduces cytokine production and bactericidal activity of human leucocytes
- Date Crossref
- 24/06/2020
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Khon Kaen University pays non établi dans la noticeUniversité ou école supérieure
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National Institute of Infectious Diseases Department of Immunology pays non établi dans la noticeOrganisme public
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London School of Hygiene & Tropical Medicine pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Associated Medical Sciences The Centre for Research and Development of Medical Diagnostic Laboratories pays non établi dans la noticeUniversité ou école supérieure
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London School of Hygiene and Tropical Medicine Department of Infection Biology pays non établi dans la noticeUniversité ou école supérieure
Khon Kaen University, Department of Immunology — National Institute of Infectious Diseases et London School of Hygiene & Tropical Medicine, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.