Blood component separation of pathogen‐reduced whole blood by the PRP method produces acceptable red cells but platelet yields and function are diminished
Rattachement africain : us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: This study evaluated blood components processed by the platelet rich plasma (PRP) method from fresh whole blood (FWB) treated with a pathogen reduction technology (PRT). The effects of storage temperature on PRT treated platelet concentrates (PCs) were also examined. STUDY DESIGN AND METHODS: PRT was performed using riboflavin and ultraviolet light on FWB in citrate phosphate dextrose anticoagulant. Following PRT, red blood cells (RBCs), PCs, and plasma for fresh frozen plasma (FFP), were isolated by sequential centrifugation. RBCs were stored at 4°C, FFP at -80°C, and PC at 22°C or at 4°C. Components were assayed throughout their storage times for blood gases, chemistry and CBC, hemostatic function as well as platelet (PLT) and RBC integrity. RESULTS: Component processing following PRT resulted in a significant drop in platelet recovery. Most PRT-PC bags fell below AABB guidelines for platelet count. PRT-PC also showed a decrease in clot strength and decreased aggregometry response. Platelet caspases were activated by PRT. Storage at 4°C improved platelet function. In PRT-FFP, prothrombin time and partial thromboplastin time (PT and aPTT) were prolonged; factors V, VII, VIII, and XI, protein C, and fibrinogen were significantly decreased. Free hemoglobin was elevated two-fold in PRT-RBC. CONCLUSION: Blood components isolated by the PRP method from PRT-treated WB result in a high percentage of PC that fail to meet AABB guidelines. FFP also shows diminished coagulation capacity. However, PRT-RBC are comparable to control-RBC. PRT-WB retains acceptable hemostatic function but alternatives to the PRP method of component separation may be more suitable.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Blood component separation of pathogen‐reduced whole blood by the PRP method produces acceptable red cells but platelet yields and function are diminished
- Date Crossref
- 01/06/2020
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
United States Army Institute of Surgical Research pays non établi dans la noticeStructure de recherche
-
Baylor University Institute of Biomedical Studies pays non établi dans la noticeUniversité ou école supérieure
-
The University of Texas at San Antonio Health Science Center pays non établi dans la noticeUniversité ou école supérieure
-
Urology San Antonio pays non établi dans la noticeÉtablissement de santé
-
The University of Texas Health Science Center at Houston pays non établi dans la noticeUniversité ou école supérieure
-
Blood Research US Army Institute of Surgical Research JBSA Fort Sam Houston Texas USA Coagulation & pays non établi dans la noticeStructure de recherche
-
Joint Interdisciplinary Biomedical Engineering Program UT San Antonio & pays non établi dans la noticeInstitution
-
Department of Surgery UT Health San Antonio San Antonio Texas USA pays non établi dans la noticeInstitution
United States Army Institute of Surgical Research, Institute of Biomedical Studies — Baylor University et The University of Texas at San Antonio Health Science Center, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.