Galangin promotes cell apoptosis through suppression of H19 expression in hepatocellular carcinoma cells
Rattachement africain : cn. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: Galangin has been extensively studied as the antitumor agent in various cancers. However, the effect of galangin in hepatocellular carcinoma (HCC) remains elusive. METHODS: Using RNA sequencing, the differential expression of lncRNA in human HCC cell line with highly metastatic potential (MHCC97H) cells treated with galangin was investigated. Furthermore, H19 expression pattern was also determined in MHCC97H cells following treatment with galangin. In addition, knockdown and overexpression of H19 was performed to analyze the effect of the expression pattern of H19 on cell apoptosis, cell cycle, migration, and invasion in HCC cells. Moreover, the in vivo effect of galangin on tumor development was also determined in nude mice. In order to analyze loss expression of H19 in vivo, clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9) was used. RESULTS: Total of 50 lncRNAs were significantly differentially expressed in MHCC97H cells treated with galangin. Besides, the expression of H19 was markedly reduced following treatment with galangin in MHCC97H cells. Compared to the Control group, the galangin-treated group inhibited cell migration and invasion. Knockdown of H19 expression showed increased cell apoptosis and decreased invasion. In addition, RNA-seq data also identified 161 mRNA which was significantly differentially expressed following treatment with galangin. To further determine the underlying mechanism, p53 protein was analyzed. Notably, the results indicated that knockdown of H19 and miR675 induced the expression of p53, eventually promoting cell apoptosis in MHCC97H cells. These results indicated that galangin promoted cell apoptosis through reduced the expression of H19 and miR675 in MHCC97H cells. The in vivo result showed that compared to the Con, tumor growth was remarkably suppressed with loss expression of H19. CONCLUSION: Our data suggested that galangin has a crucial role in hepatocarcinogenesis through regulating the expression pattern of H19.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Galangin promotes cell apoptosis through suppression of <i>H19</i> expression in hepatocellular carcinoma cells
- Date Crossref
- 02/06/2020
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Jilin University Department of Hand Surgery pays non établi dans la noticeUniversité ou école supérieure
-
First Hospital of Jilin University pays non établi dans la noticeÉtablissement de santé
-
Changchun University of Chinese Medicine Department of Pharmacy pays non établi dans la noticeUniversité ou école supérieure
-
Sun Yat-sen University pays non établi dans la noticeUniversité ou école supérieure
-
Fudan University Department of Gastroenterology and Hepatology pays non établi dans la noticeUniversité ou école supérieure
-
Zhongshan Hospital pays non établi dans la noticeÉtablissement de santé
-
College of Animal Science Laboratory Animal Center pays non établi dans la noticeUniversité ou école supérieure
Department of Hand Surgery — Jilin University, First Hospital of Jilin University et Department of Pharmacy — Changchun University of Chinese Medicine, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.