The status of the terminal regions of α-synuclein in different forms of aggregates during fibrillization
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Le résumé fourni par la source
The α-synuclein (αSN) amyloid fibrillization process is known to be a crucial phenomenon associated with neuronal loss in various neurodegenerative diseases, most famously Parkinson's disease. The process involves different aggregated species and ultimately leads to formation of β-sheet rich fibrillar structures. Despite the essential role of αSN aggregation in the pathoetiology of various neurological disorders, the characteristics of various assemblies are not fully understood. Here, we established a fluorescence-based model for studying the end-parts of αSN to decipher the structural aspects of aggregates during the fibrillization. Our model proved highly sensitive to the events at the early stage of the fibrillization process, which are hardly detectable with routine techniques. Combining fluorescent and PAGE analysis, we found different oligomeric aggregates in the nucleation phase of fibrillization with different sensitivity to SDS and different structures based on αSN termini. Moreover, we found that these oligomers are highly dynamic: after reaching peak levels during fibrillization, they decline and eventually disappear, suggesting their transformation into other αSN aggregated species. These findings shed light on the structural features of various αSN aggregates and their dynamics in synucleinopathies.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- The status of the terminal regions of α-synuclein in different forms of aggregates during fibrillization
- Date Crossref
- 01/07/2020
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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German Center for Neurodegenerative Diseases pays non établi dans la noticeStructure de recherche
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National Institute of Genetic Engineering and Biotechnology pays non établi dans la noticeStructure de recherche
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Technical University of Munich pays non établi dans la noticeUniversité ou école supérieure
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Aarhus University Institute of Industrial and Environmental Biotechnology pays non établi dans la noticeUniversité ou école supérieure
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University of Warwick pays non établi dans la noticeUniversité ou école supérieure
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School of Medicine Institute of Industrial and Environmental Biotechnology pays non établi dans la noticeUniversité ou école supérieure
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Institute of Industrial and Environmental Biotechnology Bioprocess Engineering Department pays non établi dans la noticeStructure de recherche
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Department of Translational Neurodegeneration pays non établi dans la noticeInstitution
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School of Engineering pays non établi dans la noticeUniversité ou école supérieure
German Center for Neurodegenerative Diseases, National Institute of Genetic Engineering and Biotechnology et Technical University of Munich, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.