Scn4b regulates the hypnotic effects of ethanol and other sedative drugs
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Le résumé fourni par la source
The voltage-gated sodium channel subunit β4 (SCN4B) regulates neuronal activity by modulating channel gating and has been implicated in ethanol consumption in rodent models and human alcoholics. However, the functional role for Scn4b in ethanol-mediated behaviors is unknown. We determined if genetic global knockout (KO) or targeted knockdown of Scn4b in the central nucleus of the amygdala (CeA) altered ethanol drinking or related behaviors. We used four different ethanol consumption procedures (continuous and intermittent two-bottle choice (2BC), drinking-in-the dark and chronic intermittent ethanol vapor) and found that male and female Scn4b KO mice did not differ from their wild-type (WT) littermates in ethanol consumption in any of the tests. Knockdown of Scn4b mRNA in the CeA also did not alter 2BC ethanol drinking. However, Scn4b KO mice showed longer duration of the loss of righting reflex induced by ethanol, gaboxadol, pentobarbital and ketamine. KO mice showed slower recovery to basal levels of handling-induced convulsions after ethanol injection, which is consistent with the increased sedative effects observed in these mice. However, Scn4b KO mice did not differ in the severity of acute ethanol withdrawal. Acoustic startle responses, ethanol-induced hypothermia and clearance of blood ethanol also did not differ between the genotypes. There were also no functional differences in the membrane properties or excitability of CeA neurons from Scn4b KO and WT mice. Although we found no evidence that Scn4b regulates ethanol consumption in mice, it was involved in the acute hypnotic effects of ethanol and other sedatives.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- <i>Scn4b</i> regulates the hypnotic effects of ethanol and other sedative drugs
- Date Crossref
- 02/05/2019
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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The University of Texas at Austin Waggoner Center for Alcohol and Addiction Research pays non établi dans la noticeUniversité ou école supérieure
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Scripps Research Institute Department of Neuroscience pays non établi dans la noticeOrganisation à but non lucratif
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Oregon Health & Science University pays non établi dans la noticeUniversité ou école supérieure
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University of Pittsburgh Department of Anesthesiology & pays non établi dans la noticeUniversité ou école supérieure
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University of Illinois Chicago pays non établi dans la noticeUniversité ou école supérieure
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Science University Oregon Portland Department of Behavioral Neuroscience Oregon Health & pays non établi dans la noticeUniversité ou école supérieure
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Department of Psychiatry University of Illinois at Chicago Chicago Illinois Department of Psychiatry pays non établi dans la noticeUniversité ou école supérieure
Waggoner Center for Alcohol and Addiction Research — The University of Texas at Austin, Department of Neuroscience — Scripps Research Institute et Oregon Health & Science University, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.