Three-dimensional tissue culture model of human breast cancer for the evaluation of multidrug resistance
Rattachement africain : cn, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Multidrug resistance (MDR) is one of the major obstacles to improving outcomes of chemotherapy in tumour patients. However, progress has been slow to overcome this phenomenon due to the limitations of current cell/tissue models in recapitulating MDR behaviour of tumour cells in vitro. To address this issue, a more pathologically relevant, three-dimensional (3D) culture of human breast cancer cells was developed by seeding the adriamycin-resistant cells MCF-7R in silk-collagen scaffolds. The cultures of the parental cell line MCF-7 served as controls. Distinct growth profiles of MCF-7R and MCF-7 cells were observed when they were cultured in the scaffolds in comparison with those in the monolayer culture, including cell proliferation, cellular aggregate formation, and expression of drug resistance-related genes/proteins. Moreover, the 3D cultures of these cell lines especially the cultures of MCF-7R exhibited a significantly enhanced drug resistance evidenced by their increased IC50 values to the anticancer drugs and improved drug efflux capability. An altered cell cycle distribution and improved percentage of breast cancer stem cell (BCSC)-like cells was also found in the present study. This might play an important role in promoting the drug-resistance production in those 3D cultures. Thus, we established improved 3D cultures of MDR human breast cancer. It would provide a robust tissue model for use to evaluate the efficacy of anticancer drugs, explore mechanisms of MDR, and enrich BCSCs in vitro.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Three-dimensional tissue culture model of human breast cancer for the evaluation of multidrug resistance
- Date Crossref
- 13/08/2018
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Dalian Medical University pays non établi dans la noticeUniversité ou école supérieure
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Affiliated Zhongshan Hospital of Dalian University pays non établi dans la noticeÉtablissement de santé
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Soochow University National Engineering Laboratory for Modern Silk pays non établi dans la noticeUniversité ou école supérieure
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Tufts University Department of Biomedical Engineering pays non établi dans la noticeUniversité ou école supérieure
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College of Basic Medical Science pays non établi dans la noticeUniversité ou école supérieure
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Dalian Zhongshan Hospital Affiliated Dalian University pays non établi dans la noticeUniversité ou école supérieure
Dalian Medical University, Affiliated Zhongshan Hospital of Dalian University et National Engineering Laboratory for Modern Silk — Soochow University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.