Cafestol Inhibits Cyclic‐Strain‐Induced Interleukin‐8, Intercellular Adhesion Molecule‐1, and Monocyte Chemoattractant Protein‐1 Production in Vascular Endothelial Cells
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Le résumé fourni par la source
Moderate coffee consumption is inversely associated with cardiovascular disease mortality; however, mechanisms underlying this causal effect remain unclear. Cafestol, a diterpene found in coffee, has various properties, including an anti-inflammatory property. This study investigated the effect of cafestol on cyclic-strain-induced inflammatory molecule secretion in vascular endothelial cells. Cells were cultured under static or cyclic strain conditions, and the secretion of inflammatory molecules was determined using enzyme-linked immunosorbent assay. The effects of cafestol on mitogen-activated protein kinases (MAPK), heme oxygenase-1 (HO-1), and sirtuin 1 (Sirt1) signaling pathways were examined using Western blotting and specific inhibitors. Cafestol attenuated cyclic-strain-stimulated intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein- (MCP-) 1, and interleukin- (IL-) 8 secretion. Cafestol inhibited the cyclic-strain-induced phosphorylation of extracellular signal-regulated kinase and p38 MAPK. By contrast, cafestol upregulated cyclic-strain-induced HO-1 and Sirt1 expression. The addition of zinc protoporphyrin IX, sirtinol, or Sirt1 silencing (transfected with Sirt1 siRNA) significantly attenuated cafestol-mediated modulatory effects on cyclic-strain-stimulated ICAM-1, MCP-1, and IL-8 secretion. This is the first study to report that cafestol inhibited cyclic-strain-induced inflammatory molecule secretion, possibly through the activation of HO-1 and Sirt1 in endothelial cells. The results provide valuable insights into molecular pathways that may contribute to the effects of cafestol.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Cafestol Inhibits Cyclic‐Strain‐Induced Interleukin‐8, Intercellular Adhesion Molecule‐1, and Monocyte Chemoattractant Protein‐1 Production in Vascular Endothelial Cells
- Date Crossref
- 01/01/2018
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Taipei Medical University Graduate Institute of Clinical Medicine pays non établi dans la noticeUniversité ou école supérieure
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China Medical University Department of Internal Medicine pays non établi dans la noticeUniversité ou école supérieure
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Memorial Hospital pays non établi dans la noticeÉtablissement de santé
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Institute of Biomedical Sciences pays non établi dans la noticeStructure de recherche
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China Medical University Hospital pays non établi dans la noticeÉtablissement de santé
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School of Medicine Department of Internal Medicine pays non établi dans la noticeUniversité ou école supérieure
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College of Biopharmaceutical and Food Sciences Department of Biological Science and Technology pays non établi dans la noticeUniversité ou école supérieure
Graduate Institute of Clinical Medicine — Taipei Medical University, Department of Internal Medicine — China Medical University et Memorial Hospital, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.