Micro‐computed tomography: Applications for high‐resolution skeletal density determinations: An example using annually banded crustose coralline algae
Rattachement africain : ca, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Abstract Warming and acidification of the world's oceans are expected to have widespread consequences for marine biodiversity and ecosystem functioning. However, due to the relatively short record of instrumental observations, one has to rely upon geochemical and physical proxy information stored in biomineralized shells and skeletons of calcareous marine organisms as in situ recorders of past environments. Of particular interest is the response of marine calcifiers to ocean acidification through the examination of structural growth characteristics. Here we demonstrate the application of micro‐computed tomography (micro‐CT) for three‐dimensional visualization and analysis of growth, skeletal density, and calcification in a slow‐growing, annually banded crustose coralline alga Clathromorphum nereostratum (increment width ∼380 µm). X‐ray images and time series of skeletal density were generated at 20 µm resolution and rebinned to 40, 60, 80, and 100 µm for comparison in a sensitivity analysis. Calcification rates were subsequently calculated as the product of density and growth (linear extension). While both skeletal density and calcification rates do not significantly differ at varying spatial resolutions (the latter being strongly influenced by growth rates), clear visualization of micron‐scale growth features and the quantification of structural changes on subannual time scales requires higher scanning resolutions. In the present study, imaging at 20 µm resolution reveals seasonal cycles in density that correspond to summer/winter variations in skeletal structure observed using scanning electron microscopy (SEM). Micro‐CT is a fast, nondestructive, and high‐resolution technique for structural and morphometric analyses of temporally banded paleoclimate archives, particularly those that exhibit slow or compressed growth or micron‐scale structures.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Micro‐computed tomography: Applications for high‐resolution skeletal density determinations: An example using annually banded crustose coralline algae
- Date Crossref
- 01/09/2017
- Éditeur
- American Geophysical Union (AGU)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Toronto Department of Chemical and Physical Sciences pays non établi dans la noticeUniversité ou école supérieure
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Western University pays non établi dans la noticeUniversité ou école supérieure
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Robarts Clinical Trials pays non établi dans la noticeStructure de recherche
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Robarts Research Institute pays non établi dans la noticeStructure de recherche
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National Museum of Natural History pays non établi dans la noticeInstitution
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University of Western Ontario Ontario Canada Robarts Research Institute pays non établi dans la noticeUniversité ou école supérieure
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Department of Botany pays non établi dans la noticeInstitution
Department of Chemical and Physical Sciences — University of Toronto, Western University et Robarts Clinical Trials, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.