Case of Mycobacterium haemophilum misdiagnosed as Mycobacterium intracellulare due to one base insertion in the bacterial genome
Rattachement africain : jp, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Abstract Mycobacterium haemophilum is a slow‐growing, non‐tuberculous mycobacteria that causes cutaneous infection. We describe a case of cutaneous infection in a 68‐year‐old Japanese man with polymyositis. This was caused by M. haemophilum harboring one base insertion in gene sequence. At first, the causal microorganism was misidentified as M. intracellulare by COBAS® TaqMan® MAI test. However, poor growth on Ogawa media and growth enhancement on 7H11C agar around a hemin‐containing disk prompted us to reinvestigate the causal microorganisms, which were revealed to be M. haemophilum. Amplified polymerase chain reaction products were sequenced, and the 16S rRNA gene, rpoB, hsp65 and internal transcribed spacer region sequences showed a 100%, 100%, 99.66% and 99.7% match, respectively, with the corresponding regions of M. haemophilum, but it harbored a novel gene sequence in hsp65. The sequences determined by gene analysis of the M. haemophilum strain were deposited into the International Nucleotide Sequence Database. Although numerous cases of M. haemophilum infection have been reported in other countries, only six cases have been reported in Japan to date. It could be possible that this novel mutation lead to misdiagnosis. As M. haemophilum prefers a lower growth temperature (30–32°C) and it requires iron in the culture medium, M. haemophilum could be misidentified or overlooked. Accordingly, a M. haemophilum infection should be considered in cases of cutaneous infection of the body sites, of which surface temperature is low.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Case of <i>Mycobacterium haemophilum</i> misdiagnosed as <i>Mycobacterium intracellulare</i> due to one base insertion in the bacterial genome
- Date Crossref
- 03/08/2017
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Kobe University Department of Clinical Laboratory pays non établi dans la noticeUniversité ou école supérieure
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Kobe University Hospital pays non établi dans la noticeÉtablissement de santé
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National Institutes of Health pays non établi dans la noticeOrganisme public
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National Institute of Infectious Diseases pays non établi dans la noticeOrganisme public
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Faculty of Medicine Department of Internal Related pays non établi dans la noticeUniversité ou école supérieure
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Leprosy Research Center pays non établi dans la noticeStructure de recherche
Department of Clinical Laboratory — Kobe University, Kobe University Hospital et National Institutes of Health, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.