Exogenous lysophospholipids with large head groups perturb clathrin‐mediated endocytosis
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Le résumé fourni par la source
In this study, we have investigated how clathrin-dependent endocytosis is affected by exogenously added lysophospholipids (LPLs). Addition of LPLs with large head groups strongly inhibits transferrin (Tf) endocytosis in various cell lines, while LPLs with small head groups do not. Electron and total internal reflection fluorescence microscopy (EM and TIRF) reveal that treatment with lysophosphatidylinositol (LPI) with the fatty acyl group C18:0 leads to reduced numbers of invaginated clathrin-coated pits (CCPs) at the plasma membrane, fewer endocytic events per membrane area and increased lifetime of CCPs. Also, endocytosis of Tf becomes dependent on actin upon LPI treatment. Thus, our results demonstrate that one can regulate the kinetics and properties of clathrin-dependent endocytosis by addition of LPLs in a head group size- and fatty acyl-dependent manner. Furthermore, studies performed with optical tweezers show that less force is required to pull membrane tubules outwards from the plasma membrane when LPI is added to the cells. The results are in agreement with the notion that insertion of LPLs with large head groups creates a positive membrane curvature which might have a negative impact on events that require plasma membrane invagination, while it may facilitate membrane bending toward the cell exterior.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Exogenous lysophospholipids with large head groups perturb clathrin‐mediated endocytosis
- Date Crossref
- 30/01/2017
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Oslo University Hospital Department of Molecular Cell Biology pays non établi dans la noticeÉtablissement de santé
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University of Oslo Centre for Cancer Biomedicine pays non établi dans la noticeUniversité ou école supérieure
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Norwegian University of Science and Technology Department of Physics pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Medicine Centre for Cancer Biomedicine pays non établi dans la noticeUniversité ou école supérieure
Department of Molecular Cell Biology — Oslo University Hospital, Centre for Cancer Biomedicine — University of Oslo et Department of Physics — Norwegian University of Science and Technology, avec 1 autre affiliation.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.