Comparation of Content Determination of Polysaccharides in Cuscuta Australis and Its Processed Products

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Objective:To determine content of polysaccharides of different processing drugs(plain-frying drugs,stir-heating drugs with wine and stir-heating drugs with salt) in Cuscuta australis.To explore the processing principle from the angel of changes of polysaccharides.Method:The conversion coefficient of C.australiss polysaccharides to mannitose was obtained by refined polysaccharides,and then the content of crude polysaccharides in C.australis and its processed products were determined by phenol-sulferic acid method.Result: Afer refining,monosaccharide composition of polysaccharides have no obvious changes.The color of solutions for testing showed good stabilization within 6 h and the average value of the recovery for polysaccharides measurement was 99.4% with 3.02% of RSD(n=6).The contents of polysaccharides and different processing drugs(plain-frying drugs,stir-heating drugs with wine and stir-heating drugs with salt) in C.australis were 6.71%,8.27%,8.54%,14.17%,with 0.59%,2.47%,1.40%,0.35% of RSD(n=3).Conclusion:The content of polysaccharides obviously increased after processed.Salt content is the highest;plain-frying and wine content took second place.

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