An Experimental Study on Preparing Trestle of Tissue Engineering for Skin by Collagen

Le résumé fourni par la source

Objective To build the trestle of tissue engineering for skin with collagen.Methods The collagen was obtained from the tendon of mouse tail.Its complicated manufacture involved precipitation of chondroitin-6-sulfate and collagen which were then freeze dried,dehydrathermal,cross-linked by gluteraldehyde and carefully washed.These collagen membranes were imbedded under the skin of Balb/c mice.Histological changes were observed under light microscope in 1,2,3,4 weeks postsurgery.The fibroblasts were obtained from the 3rd passaged cells,seeded into this scaffolds.Results It showed that this collagen sponge membrane was with well-distributed hole,in which bore diameter was 50~100 μm.This collagen membrane exhibited good biocompatibility and then underwent biodegradation.Conclusion The structure facilitates fibbroblasts ingrowth,suitable for building tissue engineering skin.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.