Attenuating spinal cord injury by conditioned medium from human umbilical cord blood-derived CD34+ cells in rats
Rattachement africain : tw. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
OBJECTIVE: Intravenous or intraspinal transplantation of human umbilical cord blood cells-derived CD34(+) cells (human CD34(+) cells) or mesenchymal stem cells after spinal cord injury (SCI) improved hind limb functional recovery in adult rats. The objective of this study is to ascertain whether SCI in rats can be attenuated by conditioned medium (CM) or secretome obtained from cultured human CD34(+) stem cells. MATERIALS AND METHODS: Sprague-Dawley rats were assigned to one of the following five groups: the sham group, the SCI group treated with vehicle solution (SCI + V), the SCI group treated with CM (SCI + CM), the SCI group treated with 17β-estradiol E2 (10 μg; SCI + E2), and the SCI group treated with CM plus E2 (SCI + CM + E2). A 0.5-mL volume of CM or vehicle solution was administered intravenously immediately after SCI. RESULTS: Compared with the sham group, the (SCI + V) group had significantly higher scores of neurological motor dysfunction as well as inflammation apoptosis, oxidative stress, and astrogliosis in the injured spinal cord. The neurological deficits, numbers of apoptotic cell, extent of inflammation, oxidative stress, and astrogliosis in the injured spinal cord were significantly attenuated by CM, E2, or CM plus E2, but not by the vehicle solution. In addition, the neuroprotective effect exerted by a combination of CM and E2 is superior to that exerted by CM- or E2-alone therapy. CONCLUSION: The neuroprotective effects of CM from cultured human CD34(+) cells are similar to those of human CD34(+) cells and the CM was found to enhance the neuroprotective effects of E2 in rat SCI.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Attenuating spinal cord injury by conditioned medium from human umbilical cord blood-derived CD34+ cells in rats
- Date Crossref
- 01/02/2016
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Southern Taiwan University of Science and Technology Department of Electrical Engineering pays non établi dans la noticeUniversité ou école supérieure
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Stem Cell Technology (Taiwan) pays non établi dans la noticeEntreprise
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Chi Mei Medical Center Department of Nephrology pays non établi dans la noticeÉtablissement de santé
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An-Nan Hospital pays non établi dans la noticeÉtablissement de santé
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Stem Cell Research Center pays non établi dans la noticeStructure de recherche
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Da-An Women and Children Hospital pays non établi dans la noticeÉtablissement de santé
Department of Electrical Engineering — Southern Taiwan University of Science and Technology, Stem Cell Technology (Taiwan) et Department of Nephrology — Chi Mei Medical Center, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.