Detection of somatic mutations and HPV in the saliva and plasma of patients with head and neck squamous cell carcinomas
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Le résumé fourni par la source
To explore the potential of tumor-specific DNA as a biomarker for head and neck squamous cell carcinomas (HNSCC), we queried DNA from saliva or plasma of 93 HNSCC patients. We searched for somatic mutations or human papillomavirus genes, collectively referred to as tumor DNA. When both plasma and saliva were tested, tumor DNA was detected in 96% of 47 patients. The fractions of patients with detectable tumor DNA in early- and late-stage disease were 100% (n = 10) and 95% (n = 37), respectively. When segregated by site, tumor DNA was detected in 100% (n = 15), 91% (n = 22), 100% (n = 7), and 100% (n = 3) of patients with tumors of the oral cavity, oropharynx, larynx, and hypopharynx, respectively. In saliva, tumor DNA was found in 100% of patients with oral cavity cancers and in 47 to 70% of patients with cancers of the other sites. In plasma, tumor DNA was found in 80% of patients with oral cavity cancers, and in 86 to 100% of patients with cancers of the other sites. Thus, saliva is preferentially enriched for tumor DNA from the oral cavity, whereas plasma is preferentially enriched for tumor DNA from the other sites. Tumor DNA in saliva was found postsurgically in three patients before clinical diagnosis of recurrence, but in none of the five patients without recurrence. Tumor DNA in the saliva and plasma appears to be a potentially valuable biomarker for detection of HNSCC.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Detection of somatic mutations and HPV in the saliva and plasma of patients with head and neck squamous cell carcinomas
- Date Crossref
- 24/06/2015
- Éditeur
- American Association for the Advancement of Science (AAAS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Johns Hopkins University Howard Hughes Medical Institute pays non établi dans la noticeUniversité ou école supérieure
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Sidney Kimmel Comprehensive Cancer Center pays non établi dans la noticeStructure de recherche
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Johns Hopkins Medicine pays non établi dans la noticeÉtablissement de santé
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Human Genome Sciences (United States) pays non établi dans la noticeEntreprise
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The University of Texas MD Anderson Cancer Center Department of Head and Neck Surgery pays non établi dans la noticeÉtablissement de santé
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Greater Baltimore Medical Center Milton J. Dance Head and Neck Center pays non établi dans la noticeÉtablissement de santé
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Personal Genome Diagnostics pays non établi dans la noticeInstitution
Howard Hughes Medical Institute — Johns Hopkins University, Sidney Kimmel Comprehensive Cancer Center et Johns Hopkins Medicine, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.