IMMUNOGLOBULIN Vn DETERMINANTS DEFINED BY MONOCLONAL ANTIBODIES*

Le résumé fourni par la source

The heavy and light chains of immunoglobulins (Ig) consist of two distinct regions: variable and constant. Variable regions, which determine the antibody specificity and idiotypic determinants, are further divided into three hypervariable regions and four relatively invariant, so-called framework, regions (1, 2). The variable regions of both heavy chains (VH) 1 and light chains (VL) have been divided into subgroups based upon similarities between amino acid sequences in their framework regions. For human Ig, three major VH subgroups have been characterized on the basis of amino acid sequences of the aminoterminal 20 residues within the first framework region (2). One set of goat antibodies specific for each human VH subgroup has been prepared by selective adsorption and was found to be useful in typing secreted and membranebound Ig on B cells (3). Evidence from studies (4-8) using anti-idiotype antibodies has suggested that T cells and their antigen-specific, soluble factors might possess Ig-like idiotypic determinants. VH allotypic determinants have also been demonstrable on rabbit T cells by several groups (9, 10) and not by others (11-15). Antibodies against VH framework

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