Disruption ofVisc-2, a Brain-Expressed Conserved Long Noncoding RNA, Does Not Elicit an Overt Anatomical or Behavioral Phenotype
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Le résumé fourni par la source
Although long noncoding RNAs (lncRNAs) are proposed to play essential roles in mammalian neurodevelopment, we know little of their functions from their disruption in vivo. Combining evidence for evolutionary constraint and conserved expression data, we previously identified candidate lncRNAs that might play important and conserved roles in brain function. Here, we demonstrate that the sequence and neuronal transcription of lncRNAs transcribed from the previously uncharacterized Visc locus are conserved across diverse mammals. Consequently, one of these lncRNAs, Visc-2, was selected for targeted deletion in the mouse, and knockout animals were subjected to an extremely detailed anatomical and behavioral characterization. Despite a neurodevelopmental expression pattern of Visc-2 that is highly localized to the cortex and sites of neurogenesis, anomalies in neither cytoarchitecture nor neuroproliferation were identified in knockout mice. In addition, no abnormal motor, sensory, anxiety, or cognitive behavioral phenotypes were observed. These results are important because they contribute to a growing body of evidence that lncRNA loci contribute on average far less to brain and biological functions than protein-coding loci. A high-throughput knockout program focussing on lncRNAs, similar to that currently underway for protein-coding genes, will be required to establish the distribution of their organismal functions.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Disruption of<i>Visc-2</i>, a Brain-Expressed Conserved Long Noncoding RNA, Does Not Elicit an Overt Anatomical or Behavioral Phenotype
- Date Crossref
- 10/09/2014
- Éditeur
- Oxford University Press (OUP)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Oxford Anatomy and Genetics pays non établi dans la noticeUniversité ou école supérieure
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Genomics (United Kingdom) pays non établi dans la noticeEntreprise
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National Human Genome Research Institute Embryonic Stem Cells and Transgenic Mouse Core pays non établi dans la noticeStructure de recherche
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MRC Functional Genomics Unit pays non établi dans la noticeInstitution
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Genome Technology Branch pays non établi dans la noticeInstitution
Anatomy and Genetics — University of Oxford, Genomics (United Kingdom) et Embryonic Stem Cells and Transgenic Mouse Core — National Human Genome Research Institute, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.