LSC 2012 abstract - Age-related changes in the relative expression of functional genes in mesenchymal stem cells

Le résumé fourni par la source

Age-associated changes increased susceptibility to a variety of lung pathologies. Recently, mesenchymal stem cells or B-MSCs have emerged as a critical reparative response mechanism to lung injury. We had demonstrated an association, in animal models, between age and an increase in the susceptibility to injury and we had identified functional differences between B-MSCs from young and old mice.In the present study, we examined the consequences of aging in the gene expression. METHODS: 3 and 24 months old B6 mice were sacrificed and B-MSC were isolated according to the expression of the surface markers. RNA was obtained after sorting and used for hybridization on Agilent gene expression microarrays. Ingenuity software was used to determine functional enrichment. For statistical analysis we applied significance analysis of microarrays. A qvalue of 5, which corresponds to a 5% false discovery rate, was used as a cutoff of statistical significance. Gene validations were performed by RT-PCR. RESULTS: We demonstrate that aging induce a decrease in the gene expression on B-MSC. The mechanisms affected by the decrease on gene expression includecellular trafficking, cellular growth and proliferation. CONCLUSION: Old B-MSCs have a different expression profile that exhibits a decrease in the expression of genes that control importan B-MSC functions.

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