In Vitro Efficacy of Antibiotics Commonly Used To Treat Human Plague against Intracellular Yersinia pestis
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Le résumé fourni par la source
Yersinia pestis initiates infection as a facultative intracellular parasite in host macrophages; however, little is known about the efficacy of antibiotics commonly used to treat human plague against intracellular Y. pestis. Intracellular minimal bactericidal concentrations (MBCs) were determined using a high-throughput broth microdilution assay in which human THP-1 macrophage-like cells were infected with Y. pestis strain KIM6-2053.1+ and exposed to 2-fold serial dilutions of antibiotics for 24 h in 96-well plates. The numbers of CFU, upon which minimal bactericidal concentrations were based, were determined by counting "microcolonies" in wells of 96-well plates following lysis of tissue culture cells to release surviving Y. pestis, replica dilution, and plating in soft tryptic soy broth agar. For THP-1 cells, streptomycin and ciprofloxacin had comparable efficacies for intra- and extracellular Y. pestis, but the MBCs for chloramphenicol, gentamicin, doxycycline, and amoxicillin were two-, three-, four-, and five 2-fold serial dilutions greater, respectively, for intracellular than for extracellular Y. pestis. During the initial stage of plague, intracellular Y. pestis may be less susceptible to antibiotic killing by particular antibiotics recommended for treatment of plague, such as gentamicin or doxycycline, whereas others, such as streptomycin and ciprofloxacin, may have similar efficacies against extracellular or intracellular Y. pestis. This may be of particular importance in the selection of antibiotics for prophylactic treatment in the case of a bioterrorism event.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- <i>In Vitro</i> Efficacy of Antibiotics Commonly Used To Treat Human Plague against Intracellular <i>Yersinia pestis</i>
- Date Crossref
- 01/08/2011
- Éditeur
- American Society for Microbiology
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Oklahoma State University Department of Veterinary Pathobiology pays non établi dans la noticeUniversité ou école supérieure
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United States Army Medical Command pays non établi dans la noticeOrganisme public
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United States Department of the Army pays non établi dans la noticeOrganisme public
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National Institute of Allergy and Infectious Diseases pays non établi dans la noticeStructure de recherche
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College of Veterinary Medicine pays non établi dans la noticeUniversité ou école supérieure
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Laboratory of Parasitic Diseases pays non établi dans la noticeStructure de recherche
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Merial Ltd. pays non établi dans la noticeEntreprise
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Veterinary Corps pays non établi dans la noticeInstitution
Department of Veterinary Pathobiology — Oklahoma State University, United States Army Medical Command et United States Department of the Army, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.