The Cell Surface Protein Geneecm33+Is a Target of the Two Transcription Factors Atf1 and Mbx1 and Negatively Regulates Pmk1 MAPK Cell Integrity Signaling in Fission Yeast
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Le résumé fourni par la source
The highly conserved fission yeast Pmk1 MAPK pathway plays a key role in cell integrity by regulating Atf1, which belongs to the ATF/cAMP-responsive element-binding (CREB) protein family. We identified and characterized ecm33(+), which encodes a glycosyl-phosphatidylinositol (GPI)-anchored cell surface protein as a transcriptional target of Pmk1 and Atf1. We demonstrated that the gene expression of Ecm33 is regulated by two transcription factors Atf1 and a MADS-box-type transcription factor Mbx1. We identified a putative ATF/CREB-binding site and an RLM1-binding site in the ecm33(+) promoter region and monitored the transcriptional activity of Atf1 or Mbx1 in living cells using a destabilized luciferase reporter gene fused to three tandem repeats of the CRE and six tandem repeats of the Rlm1-binding sequence, respectively. These reporter genes reflect the activation of the Pmk1 pathway by various stimuli, thereby enabling the real-time monitoring of the Pmk1 cell integrity pathway. Notably, the Deltaecm33 cells displayed hyperactivation of the Pmk1 signaling together with hypersensitivity to Ca(2+) and an abnormal morphology, which were almost abolished by simultaneous deletion of the components of the Rho2/Pck2/Pmk1 pathway. Our results suggest that Ecm33 is involved in the negative feedback regulation of Pmk1 cell integrity signaling and is linked to cellular Ca(2+) signaling.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- The Cell Surface Protein Gene<i>ecm33</i><sup>+</sup>Is a Target of the Two Transcription Factors Atf1 and Mbx1 and Negatively Regulates Pmk1 MAPK Cell Integrity Signaling in Fission Yeast
- Date Crossref
- 15/02/2010
- Éditeur
- American Society for Cell Biology (ASCB)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Kindai University pays non établi dans la noticeUniversité ou école supérieure
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Kobe University Department of Biochemistry and Molecular Biology pays non établi dans la noticeUniversité ou école supérieure
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*Laboratory of Molecular Pharmacogenomics pays non établi dans la noticeStructure de recherche
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School of Pharmaceutical Sciences Laboratory of Molecular Pharmacogenomics pays non établi dans la noticeUniversité ou école supérieure
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Laboratory of Molecular and Cellular Biology pays non établi dans la noticeStructure de recherche
Kindai University, Department of Biochemistry and Molecular Biology — Kobe University et *Laboratory of Molecular Pharmacogenomics, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.