A halotolerant thermostable lipase from the marine bacterium Oceanobacillus sp. PUMB02 with an ability to disrupt bacterial biofilms
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Le résumé fourni par la source
A halotolerant thermostable lipase was purified and characterized from the marine bacterium Oceanobacillus sp. PUMB02. This lipase displayed a high degree of stability over a wide range of conditions including pH, salinity, and temperature. It was optimally active at 30 °C and pH 8.0 respectively and was stable at higher temperatures (50-70 °C) and alkaline pH. The molecular mass of the lipase was approximately 31 kDa based on SDS-PAGE and MALDI-ToF fingerprint analysis. Conditions for enhanced production of lipase by Oceanobacillus sp. PUMB02 were attained in response surface method-guided optimization with factors such as olive oil, sucrose, potassium chromate, and NaCl being evaluated, resulting in levels of 58.84 U/ml being achieved. The biofilm disruption potential of the PUMB02 lipase was evaluated and compared with a marine sponge metagenome derived halotolerant lipase Lpc53E1. Good biofilm disruption activity was observed with both lipases against potential food pathogens such as Bacillus cereus MTCC1272, Listeria sp. MTCC1143, Serratia sp. MTCC4822, Escherichia coli MTCC443, Pseudomonas fluorescens MTCC1748, and Vibrio parahemolyticus MTCC459. Phase contrast microscopy, scanning electron microscopy, and confocal laser scanning microscopy showed very effective disruption of pathogenic biofilms. This study reveals that marine derived hydrolytic enzymes such as lipases may have potential utility in inhibiting biofilm formation in a food processing environment and is the first report of the potential application of lipases from the genus Oceanobacillus in biofilm disruption strategies.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- A halotolerant thermostable lipase from the marine bacterium <i>Oceanobacillus</i> sp. PUMB02 with an ability to disrupt bacterial biofilms
- Date Crossref
- 14/07/2014
- Éditeur
- Informa UK Limited
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Pondicherry University a Department of Food Science and Technology pays non établi dans la noticeUniversité ou école supérieure
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University College Cork pays non établi dans la noticeUniversité ou école supérieure
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Environmental Research Institute pays non établi dans la noticeOrganisation à but non lucratif
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Department of Food Science and Technology pays non établi dans la noticeInstitution
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Puducherry pays non établi dans la noticeInstitution
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Department of Microbiology pays non établi dans la noticeInstitution
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School of Microbiology and Marine Biotechnology Centre pays non établi dans la noticeUniversité ou école supérieure
a Department of Food Science and Technology — Pondicherry University, University College Cork et Environmental Research Institute, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.