Development, applications, and future prospects of RNA virus reverse genetics technology
Yu Guo, Ting Xue, Jianhua Wang, Xueqiao Xie et autres
Viral reverse genetics enables the rescue of infectious virions from cloned cDNA and serves as a core technique for mapping viral genotype–phenotype relationships, dissecting RNA viral life cycles, and developing antiviral countermeasures. This review systematically summarizes the evolution, technical framework and optimization …
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Frontiers in Virology