Wolfgang Langbein

Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6-deficient (Gata6-KOmye) mice, significant changes to the pMФ lipidome were …

ca, gb (code pays fourni par la source)

Reviewer #1 (Public review): Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6-deficient (Gata6-KOmye) mice, significant changes to the pMФ lipidome were …

Reviewer #2 (Public review): Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6-deficient (Gata6-KOmye) mice, significant changes to the pMФ lipidome were …

Reviewer #3 (Public review): Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6-deficient (Gata6-KOmye) mice, significant changes to the pMФ lipidome were …

Author response: Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6-deficient (Gata6-KOmye) mice, significant changes to the pMФ lipidome were …

Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6-deficient (Gata6-KOmye) mice, significant changes to the pMФ lipidome were …

ca, gb (code pays fourni par la source)

Genetically Encoding Stimulated Raman-Scattering Probes for Cell Imaging Using Infrared Fluorescent Proteins.

D. Regan, Ozan Aksakal, John J K McLarnon, Athena Zitti et autres

Stimulated Raman scattering (SRS) microscopy offers great potential to surpass fluorescent-based approaches, owing to the sharp line width of Raman vibrations amenable to supermultiplex cell imaging, but currently lacks one crucial component: genetically encodable tags equivalent to fluorescent proteins. Here, we show …

gb (code pays fourni par la source)

Genetic Encoding of 3-Cyano-Tyrosine and Its Use in Controlling the Chromophore Isomeric State of the Fluorescent Protein mKate

Connor J. Stevenson, John Mclarnon, James Harnedy, Salma Elsherbeni et autres

Switchable β-barrel-type fluorescent proteins are essential genetically encoded probes for super-resolution imaging. The space required for chromophore cis–trans isomerisation can also provide an opportunity to introduce bulkier chemistry at the 3-position of the phenolic ring. Here, we report, to our knowledge, the …

gb (code pays fourni par la source)

Author response for "Sub-nanometer 3D morphometric precision of polarisation-resolved wide-field optical extinction microscopy determines the roundness of individual gold nanospheres"

Lukas Payne, Furqan Alabdullah, Paola Borri, Wolfgang Langbein

Ultra-high-speed line-scan Raman imaging

Qile Wu, X W Tang, Francesco Masia, Peng Liang et autres

Raman spectroscopic imaging has emerged as a potent tool due to its non-invasive nature and capability for chemical composition analysis. Line-scan Raman spectroscopy accelerates imaging speed by two orders of magnitude compared to point detection Raman methods. However, further enhancements in imaging …

Ultra-high-speed line-scan Raman imaging

Qile Wu, X W Tang, Francesco Masia, Peng Liang et autres

Raman spectroscopic imaging has emerged as a potent tool due to its non-invasive nature and capability for chemical composition analysis. Line-scan Raman spectroscopy accelerates imaging speed by two orders of magnitude compared to point detection Raman methods. However, further enhancements in imaging …

gb, cn (code pays fourni par la source)

Comprehensive lipidomics of tissue macrophages reveal LTE4–driven eosinophil survival

Magdalena A. Czubala, Patricia Rodrigues, Natacha Ipseiz, Marcela Rosas et autres

Abstract Tissue-resident peritoneal macrophages (pMФ) programmed by GATA6 are essential players regulating immunity and tissue balance in the peritoneal cavity. How GATA6 regulates global lipid metabolism is currently unknown. Addressing this, in myeloid-restricted Gata6 -deficient (G ata6 -KO mye ) mice, significant …

ca, gb (code pays fourni par la source)