Gabriella Teti

Mapping Nanoscale Protein‐Corona Kinetics of DoE‐Optimized Perfluorocarbon Encapsulated‐PLGA Nanoparticles by In Situ, Time‐Resolved Synchrotron SAXS

Joice Maria Joseph, Maria Rosa Gigliobianco, Cristina Minnelli, Paolo Moretti et autres

ABSTRACT Integrated frameworks that couple statistically guided formulation with nanoscale, time‐resolved mapping of the protein corona (PC) remain rare for perfluoro‐15‐crown‐5‐ether‐loaded poly(lactic ‐co‐ glycolic acid) nanoparticles (PFCE‐PLGA NPs). We apply a two‐phase workflow: a single‐factor screen of PLGA/surfactant molecular‐weight ( Mw ) …

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Sulforaphane prevents oxidative stress and cell death in rat cardiomyocytes

Gabriella Teti, Cristina Angeloni, Marco Malaguti, Maria Cristina Barbalace et autres

Cardiovascular diseases (CVDs) are the major cause of death in developed countries. Oxidative stress plays a major role in the pathophysiology of cardiac disorders. Several studies have highlighted the cardinal role played by the overproduction of reactive oxygen species in the pathogenesis …

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Cobalt chloride supplementation differently affects human mesenchymal stem cells isolated from dental pulp, umbilical cord and adipose tissues in their chondrogenic potential

Gabriella Teti, Eleonora Mazzotti, Laura Ingra, Manuela Dicarlo et autres

Articular cartilage is an avascular tissue without innervations, characterized by low cell density and abundant extracellular matrix (ECM). These characteristics leave articular cartilage with very limited capacity of repair and regeneration. Multipotent stem/stromal cells (MSC) are considered promising for cartilage tissue engineering. …

Morphological and immunoistochemical evaluation of cadaveric gingival specimens to estimate the post-mortem Interval

Mirella Falconi, Paolo Fais, Lucia Manzoli, Gabriella Teti et autres

The estimation of the post-mortem interval (PMI) is a critical step in forensic medicine and remains one of the most challenging variables to determine. In general, the numerous methods currently used in estimating post-mortem interval yield to large post-mortem windows, are influenced …

3D polysaccharide based hydrogel for bone tissue engineering

Gabriella Teti, Giovanna Orsini, Antonio Mazzotti, Monica Mattioli Belmonte et autres

Hydrogels have attracted considerable attention in biomedical engineering applications due to their many favorable biomimetic properties. Hydrogels based on proteins or carbohydrates can also function as effective extracellular matrices to direct cellular behavior. Recently, polysaccharide based hydrogels have become particularly interesting as …

A synergic effect of alginate and hypoxia-inducing ions on chondrogenic differentiation in adipose derived mesenchymal stem cells

Stefano Focaroli, Viviana Salvatore, Gabriella Teti, Laura Ingrà et autres

Cartilage is a highly organized tissue with complex biomechanical properties, but since it has a poor intrinsic capacity of self-healing, injuries at this site usually lead to several problems, often ending in disabling symptoms. Although, different approach- es have been proposed, even …

Changes of microenvironment in an in vitro co-culture system

Viviana Salvatore, Gabriella Teti, Sandra Durante, Alessandra Ruggeri et autres

For the enhanced understanding of fundamental cancer biology and improving molecular diagnostics and therapeutics, the role of the microenvironment during the initiation and progression of carcinogenesis is thought to be of critical importance (1, 2). The aim of this study was to …

Influence of a biomimetic gelatin porous scaffold in chondrogenic and osteogenic differentiation of mesenchymal stem cells

Stefano Focaroli, Sandra Durante, Monica Mattioli Belmonte, Giovanna Orsini et autres

Recently, tissue engineering has merged with stem cell technology to develop new sources of transplantable material for injury or disease treatment. Eminently interesting, are bone and joint injuries/disorders because of the low self-regenerating capacity of the matrix secreting cells, particularly chondrocytes (1). …

Osteogenic differentiation of MG63 cells in biodegradable scaffolds based on gelatin and genipin

Sandra Durante, Adriana Bigi, Milena Fini, Antonio Mazzotti et autres

Gelatin is a denatured collagen and commercially available as a biodegradable polymer. It has been extensively utilized for pharmaceutical and medical purposes, and its biosafety has been proven through long clinical applications. It showed an high cell attachment and proliferation, thus it …

DMP1 and DSP localization during in vitro odontoblast like cell differentiation

Gabriella Teti, Alessandra Ruggeri, Benedetta Nicolini, Lorenzo Breschi et autres

The aim of this study was to analyze the temporal and spatial localization of dentin matrix protein 1 (DMP1) and dentin sialoprotein (DSP) in human dental pulp cells differentiated in vitro towards odontoblast-like cells. Following to a notable injury to the tooth, …

Distribution of DMP-1 and DSPP in human carious dentin: an immunohistochemical analysis

Alessandra Trirè, Viviana Salvatore, Fernando Nato, Gabriella Teti et autres

Introduction. To understand the mechanisms underlying dentin repair, several studies are focused on the analysis of the morphological aspects and chemical content of human carious dentin. Demineralization of dentin matrix causes a series of defensive reactions of the dentin-pulp complex to the …

Ultrastructure differences during in vitro osteogenesis and chondrogenesis of bone marrow mesenchymal stem cells

Mirella Falconi, Gabriella Teti, Brunella Grigolo, Carola Cavallo et autres

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