Valdir Cristóvão Barth Junior

Proteomics reveals coordinated stress adaptation by a MazF toxin to conserve carbon, sustain central metabolism, and preserve PDIM biosynthesis in Mycobacterium tuberculosis

Bruno Lopes Abbadi, Valdir Cristóvão Barth Junior, Safreen Sain, Júlia Puffal et autres

ABSTRACT In response to host-generated stresses, Mycobacterium tuberculosis (Mtb) reprograms its physiology in myriad ways to establish and maintain an infection, yet the signals that underlie this transformation are not well defined. The abundant toxin-antitoxin (TA) systems harbored in the Mtb genome, …

us, nl (code pays fourni par la source)

A Bead‐Based Screening Platform for Identifying Monoclonal Antibodies That Disrupt PD‐1/PD‐L1 Interactions

Veridiane Maria Pscheidt, Rodrigo Benedetti Gassen, José Tadeu Aranha Nunes, Deise Nascimento de Freitas et autres

Monoclonal antibodies (mAbs) targeting immune checkpoint pathways such as programmed cell death protein 1 (PD‐1)/PD‐L1 are central to modern immunotherapy, yet scalable methods to assess their functional blockade remain limited. We present a bead‐based flow cytometry assay for quantifying the inhibition of …

br (code pays fourni par la source)

Avaliação da formação de células persistentes em Acinetobacter baumannii

Valdir Cristóvão Barth Junior

A persistência é um fenótipo de tolerância a fármacos antimicrobianos com bases moleculares pouco entendidas. Tais células tolerantes (chamadas de persisters) correspondem a pequenas parcelas da população bacteriana e são capazes de sobreviver a concentrações elevadas do fármaco, mesmo sem possuir mecanismos …