G.P. Di Meo

A New and Unusual Reciprocal Translocation in Cattle: rcp(11;25)(q11;q14–21)

A. Perucatti, V. Genualdo, Alessandra Iannuzzi, L. De Lorenzi et autres

A new and unusual reciprocal translocation was detected in a heifer of the Agerolese cattle breed during a routine cytogenetic screening carried out on 13 animals (2 males and 11 females) kept at the ConSDABI Conservation Center in Benevento (Southern Italy). The …

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Copy Number Variation of Testis-Specific Protein, Y-Encoded (TSPY) in 14 Different Breeds of Cattle (Bos taurus)

Christine K. Hamilton, Laura Alessandra Favetta, G.P. Di Meo, Sandrine Floriot et autres

Multi-copied gene families are prevalent in mammalian genomes, especially within the Y chromosome. Testis specific protein Y-encoded (TSPY) is present in variable copy number in many mammalian species. Previous studies have estimated that TSPY ranges from 50-200 copies in cattle. To examine …

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Molecular Cytogenetics and Comparative Mapping in Goats (Capra hircus, 2n = 60)

Laurent Schibler, G.P. Di Meo, E.P. Cribiu, L. Iannuzzi

Few goat genome analysis projects have been developed in the last 10 years. The aim of this review was to compile and update all available cytogenetic mapping data, according to the last goat chromosome nomenclature, as well as human and cattle whole …

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X-Y Sperm Aneuploidy in 2 Cattle (Bos taurus) Breeds as Determined by Dual Color Fluorescent in situ Hybridization (FISH)

Daniel Nicodemo, Alfredo Pauciullo, Alberto Castello, Eduardo R. S. Roldán et autres

The present study was undertaken to investigate aneuploidy rates in the sperm populations of 2 cattle (Bos taurus) breeds by using dual color fluorescent in situ hybridization (FISH) with Xcen and Y chromosome-specific painting probes, obtained by chromosome microdissection and DOP-PCR. Frozen …

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FISH-mapping comparison between river buffalo chromosome 7 and sheep chromosome 6: assignment of new loci and comparison with HSA4

A. Perucatti, G.P. Di Meo, Tom Goldammer, D. Incarnato et autres

Synchronized peripheral blood lymphocytes from both river buffalo (BBU) and sheep (OAR) were treated for late incorporation of both BrdU and H-33258 to obtain R-banded preparations to be used for FISH-mapping. Ovine BAC-clones were hybridized for three days on slides pre-exposed to …

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Supplementary Material for: Molecular Cytogenetics and Comparative Mapping in Goats (Capra hircus, 2n = 60)

Laurent Schibler, G.P. Di Meo, Edmond Cribiu, L. Iannuzzi

Few goat genome analysis projects have been developed in the last 10 years. The aim of this review was to compile and update all available cytogenetic mapping data, according to the last goat chromosome nomenclature, as well as human and cattle whole …

Supplementary Material for: The 450-Band Resolution G- and R-Banded Standard Karyotype of the Donkey (Equus asinus, 2n = 62)

G.P. Di Meo, A. Perucatti, V. Peretti, D. Incarnato et autres

Donkey chromosomes were earlier characterized separately by C-, G- and R-banding techniques. However, direct comparisons between G- and R-banding patterns have still not been carried out in this species. The present study reports this comparison at the 450-band level by using replication …

High-resolution FISH mapping of β-defensin genes to river buffalo and sheep chromosomes suggests a chromosome discrepancy in cattle standard karyotypes

L. lannuzzi, D.S. Gallagher, G.P. Di Meo, Gill Diamond et autres

High-resolution chromosomal fluorescence in situ hybridization (FISH) and R-banding allowed the chromosomal localization of the beta-defensin gene cluster (DEFB@) to chromosome band 1p12 in the river buffalo (Bubalus bubalis, 2n = 50) and to band 24q13 in sheep (Ovis aries, 2n = …

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G- and R-banding comparison of sheep (Ovis aries L.) chromosomes

L. lannuzzi, G.P. Di Meo, A. Perucatti, Lino FERRARA

G- and R-banded chromosomes of 14 Gentile di Puglia sheep (Ovis aries L., 2n = 54) from southern Italy were compared at the 450-band level (ISCNDA, 1989). GTG-, GBG-, RBG-, and RBA-banding techniques were employed on slides obtained from blood cell cultures …

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Journal size increases to four volumes per year Editorial Board additions

Steve Donald Wilton, Helen J. Eyre, Patrick Anthony Akkari, Hugh C Watkins et autres

Chromosomal localization of omega and trophoblast interferon genes in cattle and river buffalo by sequential R-banding and fluorescent in situ hybridization

L. Iannuzzi, D.S. Gallagher, A. M. Ryan, G.P. Di Meo et autres

Fluorescent in situ hybridization (FISH) of the cattle cDNA probe bTP-509 to RBA-banded cattle (Bos taurus L.) chromosomes confirmed the assignment of the omega (IFNW) and trophoblast (IFNT) interferon genes to chromosome 8q15. Using the same probe, these genes were also localized …

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Molecular In Situ Hybridization Analysis of Sheep and Goat BAC Clones Identifies the Transcriptional Orientation of T Cell Receptor Gamma Genes on Chromosome 4 in Bovids

Rachele Antonacci, Giovanna Vaccarelli, G.P. Di Meo, Barbara Piccinni et autres

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