Gerhard Antoine

Detection of Minute virus of mice strains in different cell lines: Implications for adventitious agent testing

Maria R. Farcet, Jens Modrof, Gerhard Antoine, Cecilie Klausen et autres

Minute virus of mice (MMV) has contaminated biotechnological processes in the past and specific MMV testing is therefore recommended, if the production cell line is known to be permissive for this virus. Testing is widely done using cell-culture-based adventitious virus assays, yet …

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Data from Human Anti-Macrophage Migration Inhibitory Factor Antibodies Inhibit Growth of Human Prostate Cancer Cells In Vitro and In Vivo

Filza Hussain, Michael Freissmuth, Dirk Völkel, Michael Thiele et autres

Abstract Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine, originally discovered for its eponymous effect and now known for pleiotropic biologic properties in immunology and oncology. Circulating MIF levels are elevated in several types of human cancer including prostate cancer. MIF …

Supplementary Methods, Figures 1 - 3 from Human Anti-Macrophage Migration Inhibitory Factor Antibodies Inhibit Growth of Human Prostate Cancer Cells In Vitro and In Vivo

Filza Hussain, Michael Freissmuth, Dirk Völkel, Michael Thiele et autres

PDF file - 247K, Suppl. Fig. 1. Pharmacokinetics of BaxG03 after intravenous and intraperitoneal injection; Suppl. Fig. 2. Trough plasma concentrations of BaxG03 after 28 days of administration; Suppl. Fig. 3. Dose-dependent inhibition of xenograft outgrowth by BaxB01 and BaxM159.

Supplementary Methods, Figures 1 - 3 from Human Anti-Macrophage Migration Inhibitory Factor Antibodies Inhibit Growth of Human Prostate Cancer Cells In Vitro and In Vivo

Filza Hussain, Michael Freissmuth, Dirk Völkel, Michael Thiele et autres

PDF file - 247K, Suppl. Fig. 1. Pharmacokinetics of BaxG03 after intravenous and intraperitoneal injection; Suppl. Fig. 2. Trough plasma concentrations of BaxG03 after 28 days of administration; Suppl. Fig. 3. Dose-dependent inhibition of xenograft outgrowth by BaxB01 and BaxM159.

Data from Human Anti-Macrophage Migration Inhibitory Factor Antibodies Inhibit Growth of Human Prostate Cancer Cells In Vitro and In Vivo

Filza Hussain, Michael Freissmuth, Dirk Völkel, Michael Thiele et autres

Abstract Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine, originally discovered for its eponymous effect and now known for pleiotropic biologic properties in immunology and oncology. Circulating MIF levels are elevated in several types of human cancer including prostate cancer. MIF …

Optimization of an Antibody Light Chain Framework Enhances Expression, Biophysical Properties and Pharmacokinetics

Patrice Douillard, Michael Freissmuth, Gerhard Antoine, Michael Thiele et autres

Efficacy, safety, and manufacturability of therapeutic antibodies are influenced by their biopharmaceutical and biophysical properties. These properties can be optimized by library approaches or rationale protein design. Here, we employed a protein engineering approach to modify the variable domain of the light …

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A nonenveloped virus with a lipid envelope: hepatitis A virus as used in virus‐reduction studies

Anna‐Maria Kapsch, Maria R. Farcet, Gerhard Antoine, Thomas R. Kreil

BACKGROUND: Recently, a quasi-lipid-enveloped (LE) form of the traditionally nonlipid-enveloped (NLE) hepatitis A virus (HAV) was described in human serum and cell culture-derived HAV stocks. This discovery challenges the understanding of HAV reduction in virus clearance studies of plasma products, which were …

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Hepatitis E virus and the safety of plasma products: investigations into the reduction capacity of manufacturing processes

Maria R. Farcet, Cornelia Lackner, Gerhard Antoine, Philip O. Rabel et autres

BACKGROUND: Hepatitis E virus (HEV) has been transmitted by transfusion of labile blood products and the occasional detection of HEV RNA in plasma pools indicates that HEV viremic donations might enter the manufacturing process of plasma products. To verify the safety margins …

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Development of a Transgenic Mouse Model with Immune Tolerance for Human Coagulation Factor VIIa

Christine Lenk, Sabine Unterthurner, Maria Schuster, Markus Weiller et autres

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Human Anti-Macrophage Migration Inhibitory Factor Antibodies Inhibit Growth of Human Prostate Cancer Cells In Vitro and In Vivo

Filza Hussain, Michael Freissmuth, Dirk Völkel, Michael Thiele et autres

Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine, originally discovered for its eponymous effect and now known for pleiotropic biologic properties in immunology and oncology. Circulating MIF levels are elevated in several types of human cancer including prostate cancer. MIF is …

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Neutralization of Macrophage Migration Inhibitory Factor (MIF) by Fully Human Antibodies Correlates with Their Specificity for the β-Sheet Structure of MIF

Randolf J. Kerschbaumer, Manfred Rieger, Dirk Völkel, Didier Le Roy et autres

The macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that recently emerged as an attractive therapeutic target for a variety of diseases. A diverse panel of fully human anti-MIF antibodies was generated by selection from a phage display library and extensively …

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Maintenance and break of immune tolerance against human factor VIII in a new transgenic hemophilic mouse model

Pauline M. van Helden, Sabine Unterthurner, Corinna Hermann, Maria Schuster et autres

Replacement of the missing factor VIII (FVIII) is the current standard of care for patients with hemophilia A. However, the short half-life of FVIII makes frequent treatment necessary. Current efforts focus on the development of longer-acting FVIII concentrates by introducing chemical and …

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