María Ángeles Trillo

Field exposure to 50 Hz significantly affects wild‑type and unfolded p53 expression in NB69 neuroblastoma cells

Juan Carlos Martín, Alejandro Úbeda, Javier Martı́nez-Botas, María Ángeles Trillo

Previous studies have shown that intermittent exposure to a 50 Hz, 100 µT sinusoidal magnetic field (MF) promotes proliferation of human neuroblastoma cells, NB69. This effect is mediated by activation of the epidermal growth factor receptor through a free radical-dependent activation of …

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Response of human cancer cells to simultaneous treatment with sorafenib and radiofrequency current

María Luisa Hernández‐Bule, María Antonia Martínez, María Ángeles Trillo, Lidia Martínez et autres

Due to their alleged analgesic, anti-inflammatory and tissue regenerative effects, capacitive-resistive electrothermal therapy (CRET), which is based on non-invasive exposure to radiofrequency (RF) currents, is often applied to chemotherapeutically treated patients with cancer. Our previous studies have demonstrated that subthermal CRET currents …

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Role of NADPH oxidase in MAPK signaling activation by a 50 Hz magnetic field in human neuroblastoma cells

María Antonia Martínez, Alejandro Úbeda, María Ángeles Trillo

Our previous studies have shown that intermittent exposure to a 50-Hz, 100-µT sine wave magnetic field (MF) promotes human NB69 cell proliferation, mediated by activation of the epidermal growth factor receptor (EGFR) and pathways MAPK-ERK1/2 and p38; being the effects on proliferation …

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Effects of the signal modulation on the response of human fibroblasts to in vitro stimulation with subthermal RF currents

María Ángeles Trillo, María Antonia Martínez, Alejandro Úbeda

Capacitive-Resistive Electric Transfer (CRET) thermotherapies aim at tissue repair and regeneration through non-invasive application of RF currents. We have reported that the cellular response to subthermal CRET currents is non-linearly dependent on the signal frequency, and that in vitro exposure to a …

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Stem Cell Research 2018-Chondrogenic Differentiation of Adipose-Derived Stem Cells by Radiofrequency Electric Stimulation- Maria Luisa Hernandez-Bule- Ramon y Cajal University Hospital

María Luisa Hernández‐Bule, María Ángeles Trillo, María Ángeles Martínez-García, Carlos Abilahoud

Introduction: There is ample evidence that stimulation with electric currents and electric and/or magnetic fields can induce a variety of cellular and molecular responses, including microfilament reorganization, redistribution of cell surface receptors or cell migration, as well as changes in intracellular calcium …

Involvement of the EGF Receptor in MAPK Signaling Activation by a 50 Hz Magnetic Field in Human Neuroblastoma Cells

María Antonia Martínez, Alejandro Úbeda, María Ángeles Trillo

BACKGROUND/AIMS: Previous studies have shown that a 63-hour, intermittent exposure to a 50 Hz, 100 μT magnetic field (MF) induces in the NB69 line of human neuroblastoma a proliferative response that is mediated by activation of the MAPK pathways ERK1/2 and p38. …

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Chondrogenic Differentiation of Adipose-Derived Stem Cells by Radiofrequency Electric Stimulation

María Luisa Hernández‐Bule, María Ángeles Trillo, María Ángeles Martínez-García, Carlos Abilahoud et autres

Objective: Although capacitive-resistive electric transfer (CRET) therapies, based on transdermal application of electrothermal radiofrequency currents, have shown promising therapeutic effectiveness in regeneration of traumatic or degenerative tissue lesions, their potential effects on tissues like cartilage, having poor regenerative capabilities, have not been …

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Power Frequency Magnetic Fields Affect the p38 MAPK-Mediated Regulation of NB69 Cell Proliferation Implication of Free Radicals

Juan Carlos Martín, Alejandro Úbeda, Jorge Moreno, María Ángeles Trillo

The proliferative response of the neuroblastoma line NB69 to a 100 µT, 50 Hz magnetic field (MF) has been shown mediated by activation of the MAPK-ERK1/2 pathway. This work investigates the MF effect on the cell cycle of NB69, the participation of …

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Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells

María Luisa Hernández‐Bule, Javier Martı́nez-Botas, María Ángeles Trillo, Carlos Luis Paı́no et autres

The 448 kHz capacitive‑resistive electric transfer (CRET) is an electrothermal therapy currently applied in anticellulite and antiobesity treatments. The aim of the present study was to determine whether exposure to the CRET electric signal at subthermal doses affected early adipogenic processes in …

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Power-Frequency Magnetic Field Inhibits Adipogenic Differentiation in Human ADSC

María Antonia Martínez, María Ángeles Trillo, María Antonia Cid, Carlos Luis Paı́no et autres

BACKGROUND/AIMS: Semicircular lipoatrophy (SL) is an idiopathic condition characterized by atrophy of subcutaneous fatty tissue. Although several studies have suggested a possible association between SL and occupational exposure to power frequency magnetic fields (MF), no mechanism has been proposed so far that …

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Molecular Mechanisms Underlying Antiproliferative and Differentiating Responses of Hepatocarcinoma Cells to Subthermal Electric Stimulation

María Luisa Hernández‐Bule, María Ángeles Trillo, Alejandro Úbeda

Capacitive Resistive Electric Transfer (CRET) therapy applies currents of 0.4-0.6 MHz to treatment of inflammatory and musculoskeletal injuries. Previous studies have shown that intermittent exposure to CRET currents at subthermal doses exert cytotoxic or antiproliferative effects in human neuroblastoma or hepatocarcinoma cells, …

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Electric Stimulation at 448 kHz Promotes Proliferation of Human Mesenchymal Stem Cells

María Luisa Hernández‐Bule, Carlos Luis Paı́no, María Ángeles Trillo, Alejandro Úbeda

BACKGROUND/AIMS: Capacitive-resistive electric transfer (CRET) is a non invasive electrothermal therapy that applies electric currents within the 400 kHz - 450 kHz frequency range to the treatment of musculoskeletal lesions. Evidence exists that electric currents and electric or magnetic fields can influence …

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