Inhwan Hwang

Shigella Effector VirA Suppresses Plant Immunity by Compromising PRA1.F3‐Dependent Accumulation of FLS2 at the Plasma Membrane

Sung Hee Jo, Sukyoung Jung, Myoung Hui Lee, Inhwan Hwang et autres

Surface immune receptors such as flagellin-sensitive 2 (FLS2) are critical for plant defence, and their accumulation at the plasma membrane is tightly controlled by the endomembrane system. Here, we identify the Arabidopsis thaliana prenylated Rab acceptor PRA1.F3 as a positive regulator required …

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Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in Nicotiana benthamiana

Hai‐Ping Diao, Hao Meng, Xuejiao Xu, Zeng‐Lin Zhang et autres

Efficient purification remains a key technical challenge affecting the recovery efficiency of recombinant proteins in plant-based systems. Complex metabolites, particularly polyphenols, often cause recombinant protein aggregation during purification. In this study, we identified two key polyphenol oxidase genes, PPOa and PPOb from …

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Chromosome-level genome assembly of Bienertia sinuspersici, Single-cell-C4 plant

Jung Sun Kim, Seung Jae Lee, Ho Young Chung, Inhwan Hwang et autres

Bienertia sinuspersici is a flowering plant currently classified under the family Amaranthaceae. It is an important C 4 model plant with dimorphic chloroplast on single chlorenchyma cells. Subcellular-compartmentalization of peripheral compartment chloroplast (PCC) and central compartment chloroplast (CCC) in the single cell …

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Natural peptidoglycan nanoparticles enable rapid antigen purification and potent delivery of plant-derived vaccines

Shi‐Jian Song, Hai‐Ping Diao, Sensen Zhang, Sumin Kang et autres

Plant molecular farming is a promising platform for biopharmaceutical production, however, downstream processing remains a challenge due to cost and complexity. In this study, we present natural peptidoglycan nanoparticles (NPNs) derived from Gram-positive lactic acid bacteria as a novel tool for plant-based …

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Development of Plant-Based Multivalent Vaccine Candidates for SARS-CoV-2 and Influenza Virus Using Inactivated Lactococcus

Dong-Sook Lee, Hasanul Banna, Heeyeon Kim, Md Rezaul Islam Khan et autres

Background/Objectives: Since December 2019, the COVID-19 pandemic, driven by SARS-CoV-2, has caused ~690 million infections globally, manifesting with mild to severe symptoms, including pneumonia. After reduced activity, seasonal influenza re-emerged in winter 2022, creating a “twindemic” with SARS-CoV-2. Co-infections have been associated …

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Expression and localization of two β-carbonic anhydrases in Bienertia, a single-cell C4 plant

Phu-Tho Nguyen, N.-E. Lee, Fabian Frömling, Toni Luise Meister et autres

Carbonic anhydrases (CAs) are ubiquitous enzymes that catalyze reversibly both the hydration and dehydration reactions of CO2 and HCO3-, respectively. Higher plants contain many different isoforms of CAs that can be classified into α-, β- and γ-type subfamilies. β-type CAs play a …

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Developing a hybrid carbonic anhydrase with exceptional high temperature and alkaline environments resistance for efficient CO2 capture from air

Madhu Kumari, Aditya Prakash Soni, Bongryul Ryu, Inseop Chun et autres

Reducing atmospheric CO2 levels to combat global warming is a pressing concern today. Numerous methods have been employed to capture CO2 from flue gases. One particularly promising approach is the use of carbonic anhydrases (CAs) as biocatalysts for the rapid conversion of …