Akira Nakamura

Global identification of highly expandable and shrinkable proteins via intramolecular distance scoring analysis

Tetsuji Okada, Fumiaki Tomoike, Takeshi Itabashi, T. Nagae et autres

Abstract Experimental and analytical characterizations of protein structural changes are essential for understanding various biological functions. It is well known that each protein or protein complex undergoes shape changes in a specific manner and to a certain extent, influenced by factors such …

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Structural Analysis of Phosphonopyruvate Decarboxylase RhiEF: First Insights into an Ancestral Heterooligomeric Thiamine Pyrophosphate-Dependent Decarboxylase

Akira Nakamura, Ayaka Shiina, Shuichi Kojima

The RhiE and RhiF proteins work together as RhiEF and function as a thiamine pyrophosphate (TPP)-dependent phosphonopyruvate decarboxylase to produce phosphonoacetaldehyde in the rhizocticin biosynthesis pathway. In this study, we determined the crystal structure of the RhiEF complexed with TPP and Mg …

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Random mutagenesis and disulfide bond formation improved thermostability in microbial transglutaminase

Mototaka Suzuki, Masayo Date, Tatsuki Kashiwagi, Kazutoshi Takahashi et autres

Microbial transglutaminase (MTG) from Streptomyces mobaraensis is widely used in the food and pharmaceutical industries for cross-linking and post-translational modification of proteins. It is believed that its industrial applications could be further broadened by improving its thermostability. In our previous study, we …

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COVID-19 vaccine effectiveness against severe COVID-19 requiring oxygen therapy, invasive mechanical ventilation, and death in Japan: A multicenter case-control study (MOTIVATE study)

Takeshi Arashiro, Maki Miwa, Hidenori Nakagawa, Junpei Takamatsu et autres

INTRODUCTION: Since the SARS-CoV-2 Omicron variant became dominant, assessing COVID-19 vaccine effectiveness (VE) against severe disease using hospitalization as an outcome became more challenging due to incidental infections via admission screening and variable admission criteria, resulting in a wide range of estimates. …

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Crystal structure of the AlbEF complex involved in subtilosin A biosynthesis

Kohki Ishida, Akira Nakamura, Shuichi Kojima

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Development of a novel peptide inhibitor of subtilisin BPN ′

Kohki Ishida, Makoto Shimizu, Ayumi Wakasugi, Yuko Matsui et autres

Proteinaceous protease inhibitors can strongly and specifically inhibit cognate proteases, but their use as pharmaceuticals is limited by their size. As such, the development of effective protease peptide inhibitors would be beneficial for biochemical studies and drug discovery. In this study, we …

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Highlighting the potential utility of MBP crystallization chaperone for Arabidopsis BIL1/BZR1 transcription factor-DNA complex

Shohei Nosaki, Tohru Terada, Akira Nakamura, Kei Hirabayashi et autres

The maltose-binding protein (MBP) fusion tag is one of the most commonly utilized crystallization chaperones for proteins of interest. Recently, this MBP-mediated crystallization technique was adapted to Arabidopsis thaliana (At) BRZ-INSENSITIVE-LONG (BIL1)/BRASSINAZOLE-RESISTANT (BZR1), a member of the plant-specific BZR TFs, and revealed …

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Identification of novel interacting regions involving calcineurin and nuclear factor of activated T cells

Noriko Kitamura, Mayumi Shindo, Jun Ohtsuka, Akira Nakamura et autres

Abstract Nuclear factor of activated T cells (NFAT) leads to the transcription of diverse inducible genes involved in many biological processes; therefore, aberrant NFAT expression is responsible for the development and exacerbation of various disorders. Since five isoforms of NFAT (NFATc1‐c4, NFAT5) …

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Methylene Blue Inhibits Formation of Tau Fibrils but not of Granular Tau Oligomers: A Plausible Key to Understanding Failure of a Clinical Trial for Alzheimer’s Disease

Yoshiyuki Soeda, Marino Saito, Sumihiro Maeda, Kohki Ishida et autres

Alzheimer's disease pathology is characterized by extracellular deposits of amyloid-β (Aβ) and intracellular inclusions of hyperphosphorylated tau. Although genetic studies of familial Alzheimer's disease suggest a causal link between Aβ and disease symptoms, the failure of various Aβ-targeted strategies to slow or …

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Evaluation of Dynamic Tumor-tracking Intensity-modulated Radiotherapy for Locally Advanced Pancreatic Cancer

Akira Nakamura, Masahiro Hiraoka, Satoshi Itasaka, Mitsuhiro Nakamura et autres

Intensity-modulated radiotherapy (IMRT) is now regarded as an important treatment option for patients with locally advanced pancreatic cancer (LAPC). To reduce the underlying tumor motions and dosimetric errors during IMRT as well as the burden of respiratory management for patients, we started …

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Structural basis for brassinosteroid response by BIL1/BZR1

Shohei Nosaki, Takuya Miyakawa, Yuqun Xu, Akira Nakamura et autres

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Structural analysis of HTL and D14 proteins reveals the basis for ligand selectivity in Striga

Yuqun Xu, Takuya Miyakawa, Shohei Nosaki, Akira Nakamura et autres

HYPOSENSITIVE TO LIGHT (HTL) and DWARF14 (D14) mediate the perception of karrikin and strigolactone, which stimulates germination of the parasitic weed Striga. However, their role in parasitic seeds is poorly understood, and the basis for their differing responsiveness remains unclear. Here, we …

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