Accès ouvert
2026
preprint
OpenAlex
Victor Mabasa, Natasha Singh, Emmanuel Phalane, Sipho Gwala et autres
This protocol is optimised for quantifying RNA and DNA targets using the QIAcuity OneStep Advanced Probe Kit with hydrolysis probes in singleplex or multiplex (up to two targets) reactions on QIAGEN’s QIAcuity digital PCR (dPCR) instruments. It also includes instructions for preparing …
Accès ouvert
2026
article
OpenAlex
Zinhle E. Mthombothi, Adrian Lison, Fiona Els, Cari van Schalkwyk et autres
Inferring epidemic trajectories of viral infections from wastewater data can be a useful addition to clinical-based surveillance, as it provides low cost, population-level data that includes both symptomatic and asymptomatic individuals who contribute to the sewer system. However, methods for analyzing wastewater …
Afrique du Sud, ch, gb
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Zinhle E. Mthombothi, Adrian Lison, Fiona Els, Cari van Schalkwyk et autres
Inferring epidemic trajectories of viral infections from wastewater data can be a useful addition to clinical-based surveillance, as it provides low cost, population-level data that includes both symptomatic and asymptomatic individuals who contribute to the sewer system. However, methods for analyzing wastewater …
Accès ouvert
2026
preprint
OpenAlex
Victor Vusi Mabasa, Natasha Singh, Emmanuel Phalane, Sipho Gwala et autres
This protocol is optimised for quantifying RNA and DNA targets using the QIAcuity OneStep Advanced Probe Kit with hydrolysis probes in singleplex or multiplex (up to two targets) reactions on QIAGEN’s QIAcuity digital PCR (dPCR) instruments. It also includes instructions for preparing …
Afrique du Sud, mn
(code pays fourni par la source)
Accès ouvert
2026
preprint
OpenAlex
Sipho Gwala, Joshua I. Levy, Victor Vusi Mabasa, Kathleen Subramoney et autres
ABSTRACT Measles outbreaks have surged globally in recent years, but current surveillance systems have limited capacity to monitor measles virus (MeV) transmission and evolution at population scale. Although MeV can be detected in wastewater, the public health potential of wastewater genomic surveillance …
Afrique du Sud, us, gb
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Zinhle E. Mthombothi, Adrian Lison, Fiona Els, Cari van Schalkwyk et autres
Inferring epidemic trajectories of viral infections from wastewater data can be a useful addition to clinical-based surveillance, as it provides low cost, population-level data that includes both symptomatic and asymptomatic individuals who contribute to the sewer system. However, methods for analyzing wastewater …
Afrique du Sud, ch, gb
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Victor Vusi Mabasa, Natasha Singh, Emmanuel Phalane, Mokgaetji Macheke et autres
This wastewater sample processing protocol by the Wastewater Genomics Syndicate at the National Institute of Communicable Diseases (NICD) is designed to enrich bacteria and viruses from the settled solids and supernatant components of wastewater, as different pathogens partition differently within this matrix. …
us, mn
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Victor Vusi Mabasa, Natasha Singh, Emmanuel Phalane, Sipho Gwala et autres
This protocol is optimised for quantifying RNA and DNA targets using the QIAcuity OneStep Advanced Probe Kit with hydrolysis probes in singleplex or multiplex (up to two targets) reactions on QIAGEN’s QIAcuity digital PCR (dPCR) instruments. It also includes instructions for preparing …
Afrique du Sud, mn
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Nosihle S Msomi, Joshua I. Levy, Nathaniel L. Matteson, Nkosenhle Lindo Ndlovu et autres
Timely pathogen surveillance and reporting is essential for effective public health guidance. Web dashboards have become a key tool for communicating public health information to stakeholders, health care workers, and the broader community. Over the SARS-CoV-2 pandemic, wastewater surveillance has increasingly been …
Afrique du Sud, us
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Victor Vusi Mabasa, Natasha Singh, Emmanuel Phalane, Sipho Gwala et autres
This protocol utilises Ceres Nanotrap beads and Dynabeads for virus enrichment from 20 mL wastewater samples. The Applied Biosystems MagMAX Wastewater Ultra Nucleic Acid Isolation Kit (Cat. No. A52610) is then employed for total nucleic acid extraction. The purified nucleic acids are …
us
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Victor Vusi Mabasa, Natasha Singh, Emmanuel Phalane, Mokgaetji Macheke et autres
The Applied Biosystems MagMAX Wastewater Ultra Nucleic Acid Isolation Kit (Cat. No. A52610) is specifically designed for virus enrichment and total nucleic acid extraction from 10 mL wastewater samples. The purified nucleic acids suit various downstream applications, including real-time PCR, digital PCR, …
mn
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Nosihle S Msomi, Joshua I. Levy, Nathaniel L. Matteson, Nkosenhle Lindo Ndlovu et autres
ABSTRACT Timely pathogen surveillance and reporting is essential for effective public health guidance. Web dashboards have become a key tool for communicating public health information to stakeholders, health care workers, and the broader community. Over the SARS-CoV-2 pandemic, wastewater surveillance has increasingly …
Afrique du Sud, us
(code pays fourni par la source)