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Profil bibliographique

Emmanuel Phalane

Informations fournies par OpenAlex. Research Africa ne déduit ni nationalité, ni poste, ni coordonnées personnelles.

15Publications signalées
21Citations signalées
3Affiliations récentes

Les institutions déclarées

Les domaines associés

SARS-CoV-2 detection and testingCOVID-19 epidemiological studiesFecal contamination and water qualityBiosensors and Analytical DetectionMolecular Biology Techniques and Applications

Les publications récentes

Accès ouvert 2026 article OpenAlex

Evaluation and estimation of epidemic trajectories for SARS-CoV-2 from clinical and wastewater data in Gauteng Province, South Africa

Zinhle E. Mthombothi, Adrian Lison, Fiona Els, Cari van Schalkwyk et autres

Inferring epidemic trajectories of viral infections from wastewater data can be a useful addition to clinical-based surveillance, as it provides low cost, population-level data that includes both symptomatic and asymptomatic individuals who contribute to the sewer system. However, methods for analyzing wastewater …

Afrique du Sud, ch, gb (code pays fourni par la source)

0 citations PLOS Global Public Health
Accès ouvert 2026 article OpenAlex

Evaluation and estimation of epidemic trajectories for SARS-CoV-2 from clinical and wastewater data in Gauteng Province, South Africa

Zinhle E. Mthombothi, Adrian Lison, Fiona Els, Cari van Schalkwyk et autres

Inferring epidemic trajectories of viral infections from wastewater data can be a useful addition to clinical-based surveillance, as it provides low cost, population-level data that includes both symptomatic and asymptomatic individuals who contribute to the sewer system. However, methods for analyzing wastewater …

0 citations Repository for Publications and Research Data (ETH Zurich)
Accès ouvert 2026 preprint OpenAlex

Multi-pathogen detection & quantification using digital PCR v2

Victor Vusi Mabasa, Natasha Singh, Emmanuel Phalane, Sipho Gwala et autres

This protocol is optimised for quantifying RNA and DNA targets using the QIAcuity OneStep Advanced Probe Kit with hydrolysis probes in singleplex or multiplex (up to two targets) reactions on QIAGEN’s QIAcuity digital PCR (dPCR) instruments. It also includes instructions for preparing …

Afrique du Sud, mn (code pays fourni par la source)

0 citations
Accès ouvert 2026 preprint OpenAlex

Integrating measles wastewater and clinical whole-genome sequencing enables high-resolution tracking of virus evolution and transmission

Sipho Gwala, Joshua I. Levy, Victor Vusi Mabasa, Kathleen Subramoney et autres

ABSTRACT Measles outbreaks have surged globally in recent years, but current surveillance systems have limited capacity to monitor measles virus (MeV) transmission and evolution at population scale. Although MeV can be detected in wastewater, the public health potential of wastewater genomic surveillance …

Afrique du Sud, us, gb (code pays fourni par la source)

1 citation medRxiv
Accès ouvert 2026 article OpenAlex

Evaluation and estimation of epidemic trajectories for SARS-CoV-2 from clinical and wastewater data in Gauteng Province, South Africa.

Zinhle E. Mthombothi, Adrian Lison, Fiona Els, Cari van Schalkwyk et autres

Inferring epidemic trajectories of viral infections from wastewater data can be a useful addition to clinical-based surveillance, as it provides low cost, population-level data that includes both symptomatic and asymptomatic individuals who contribute to the sewer system. However, methods for analyzing wastewater …

Afrique du Sud, ch, gb (code pays fourni par la source)

0 citations LSHTM Research Online (London School of Hygiene and Tropical Medicine)
Accès ouvert 2025 preprint OpenAlex

Clarification of wastewater samples v1

Victor Vusi Mabasa, Natasha Singh, Emmanuel Phalane, Mokgaetji Macheke et autres

This wastewater sample processing protocol by the Wastewater Genomics Syndicate at the National Institute of Communicable Diseases (NICD) is designed to enrich bacteria and viruses from the settled solids and supernatant components of wastewater, as different pathogens partition differently within this matrix. …

us, mn (code pays fourni par la source)

0 citations
Accès ouvert 2025 preprint OpenAlex

Multi-pathogen detection & quantification using digital PCR v1

Victor Vusi Mabasa, Natasha Singh, Emmanuel Phalane, Sipho Gwala et autres

This protocol is optimised for quantifying RNA and DNA targets using the QIAcuity OneStep Advanced Probe Kit with hydrolysis probes in singleplex or multiplex (up to two targets) reactions on QIAGEN’s QIAcuity digital PCR (dPCR) instruments. It also includes instructions for preparing …

Afrique du Sud, mn (code pays fourni par la source)

1 citation
Accès ouvert 2025 article OpenAlex

Wastewater-integrated pathogen surveillance dashboards enable real-time, transparent, and interpretable public health risk assessment and dissemination

Nosihle S Msomi, Joshua I. Levy, Nathaniel L. Matteson, Nkosenhle Lindo Ndlovu et autres

Timely pathogen surveillance and reporting is essential for effective public health guidance. Web dashboards have become a key tool for communicating public health information to stakeholders, health care workers, and the broader community. Over the SARS-CoV-2 pandemic, wastewater surveillance has increasingly been …

Afrique du Sud, us (code pays fourni par la source)

7 citations PLOS Global Public Health
Accès ouvert 2025 preprint OpenAlex

Ceres Nanotrap & Dynabeads Combined Wastewater Virus Enrichment & Total Nucleic Acid Extraction v1

Victor Vusi Mabasa, Natasha Singh, Emmanuel Phalane, Sipho Gwala et autres

This protocol utilises Ceres Nanotrap beads and Dynabeads for virus enrichment from 20 mL wastewater samples. The Applied Biosystems MagMAX Wastewater Ultra Nucleic Acid Isolation Kit (Cat. No. A52610) is then employed for total nucleic acid extraction. The purified nucleic acids are …

us (code pays fourni par la source)

1 citation
Accès ouvert 2025 preprint OpenAlex

Dynabeads Wastewater Virus Enrichment & Total Nucleic Acid Extraction v1

Victor Vusi Mabasa, Natasha Singh, Emmanuel Phalane, Mokgaetji Macheke et autres

The Applied Biosystems MagMAX Wastewater Ultra Nucleic Acid Isolation Kit (Cat. No. A52610) is specifically designed for virus enrichment and total nucleic acid extraction from 10 mL wastewater samples. The purified nucleic acids suit various downstream applications, including real-time PCR, digital PCR, …

mn (code pays fourni par la source)

0 citations
Accès ouvert 2025 preprint OpenAlex

Wastewater-integrated pathogen surveillance dashboards enable real-time, transparent, and interpretable public health risk assessment and dissemination

Nosihle S Msomi, Joshua I. Levy, Nathaniel L. Matteson, Nkosenhle Lindo Ndlovu et autres

ABSTRACT Timely pathogen surveillance and reporting is essential for effective public health guidance. Web dashboards have become a key tool for communicating public health information to stakeholders, health care workers, and the broader community. Over the SARS-CoV-2 pandemic, wastewater surveillance has increasingly …

Afrique du Sud, us (code pays fourni par la source)

2 citations medRxiv

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