Yaoyi Zhang

Input-Triggered Allosteric Activation of CRISPR–Cas12a by Modular Loop-Engineered Hairpins

Na Yin, Li Zhang, Ruiling Lu, Hongyan Yu et autres

Achieving precise control over CRISPR-Cas12a activity remains a fundamental challenge in the development of versatile sensing platforms, particularly for applications in diagnostics. Herein, we report an allosteric strategy that employs modular loop-engineered hairpin (MLEH) to precisely control Cas12a activation, thereby establishing a …

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CAMERA: A programmable CRISPR-gated logic system for high-fidelity assessment of Mycobacterium tuberculosis

Jiu Pu, Dan Bai, Yaoyi Zhang, Yiqi Zhang et autres

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A structural accessibility principle for LbuCas13a activation by noncontiguous DNA

W Y Wang, Yuhan Chen, Z Li, Li Zhang et autres

CRISPR-Cas13a is mainly known as an RNA-guided RNA endonuclease. Recent studies show that Leptotrichia buccalis Cas13a (LbuCas13a) can interact with DNA substrates too, without PAM or PFS constraints, but current understanding of DNA-mediated activation is largely based on continuous target strands. Here, …

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The Comparison of Ionic Aggregate Structure and Structural Morphology of Polyether‐Ester‐Sulfonate Copolymer Ionomers With Lithium and Sodium Counterions

Jianchuan Liu, Jiaxin Song, Yaoyi Zhang, S S Wang et autres

ABSTRACT Using all‐atom molecular dynamics simulations, this paper investigates the differences in ionic aggregate structure and structural morphology of polyether‐ester sulfonate copolymers (containing poly(ethylene oxide) (PEO) and poly(tetramethylene oxide) (PTMO)) with Li + and Na + as counterions. Compared with the 100% …

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Programmable pH-responsive DNA inter-strand matching (PRISM) for precision molecular band-pass actuation

Xiaole Han, Hongyan Yu, Xiaomei Lin, Li Zhang et autres

Background: Precise sensing of narrow physiological pH ranges is important for accurate molecular regulation in complex biological environments. Traditional pH-responsive systems generally rely on monotonic off-to-on switching, making it difficult to distinguish subtle differences in tumor microenvironment pH (6.5-6.8) from neutral healthy …

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Detachable TMSD-CRISPR for programmable and highly specific discrimination of DNA/RNA mutations

Y Y Zhang, Wang Luo, Yaoyi Zhang, Y F He et autres

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A universal and orthogonal safety valve for CRISPR/Cas12a without chemical modification or external stimulation

Wang Luo, Y J Wu, Dongsheng Ni, Li Zhang et autres

CRISPR/Cas-based gene editing technologies have achieved remarkable progress over the past decade, yet their broad practical applications remain limited by safety concerns. Although regulatory strategies applied before or during CRISPR/Cas activation have substantially improved sequence, temporal, and spatial specificity, persistent activity of …

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Coarse‐Grained Molecular Dynamics Study on Naphthalene‐Based Solid Additives for Optimizing Morphology in PM6: PYIT Organic Solar Cells

Jianchuan Liu, Yaoyi Zhang, Jiaxin Song, Shun Wang et autres

ABSTRACT The use of solid additives is crucial for optimizing blend morphology and enhancing power conversion efficiency (PCE) in organic photovoltaics (OPVs). This study investigates four naphthalene‐derived additives (2‐chloronaphthalene (2‐CN), 2‐methylnaphthalene (2‐MN), 2‐methylthionaphthalene (2‐sMN), and 2‐methoxynaphthalene (2‐oMN)) in PM6:PYIT blends via coarse‐grained …

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Molecular Insights into the Reversible Mechanisms of CO2-Switchable Surfactant Solubilization and Emulsification

Jianchuan Liu, Jiaxin Song, Yaoyi Zhang, Xinyue Yu et autres

Switchable surfactants exhibit broad application potential due to their reversible response to external stimuli. The reversible mechanism of the CO 2 -switchable surfactant ( N ’-dodecyl- N, N -dimethyl-acetamidines, DDA) solubilization polycyclic aromatic hydrocarbons (PAHs) and the microscopic dynamic behavior of emulsification/demulsification …

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Rational design of tunable pH switches through shadow-strand hybridization-actuated displacement engineering

Xiaole Han, Xiangyu Dong, Xiaomei Lin, Hongyan Yu et autres

Local pH variations play a pivotal role in numerous critical biological processes. However, achieving the tunability and selectivity of pH detection remains a challenge. Here, we present a DNA-based strategy that enables programmable and selective pH responses, which is termed shadow-strand hybridization-actuated …

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Logical Analysis of Multiple miRNAs with Isothermal Molecular Classifiers Based on LATE-RCA

Tong Feng, Li Zhang, Ding Wang, Zuo Chen et autres

Logical analysis of multiple-miRNA expression information and immediate output of diagnostic results facilitates early cancer detection. In this work, we constructed an isothermal molecular classifier capable of performing computations on multiple miRNAs and directly providing diagnosis results. First, we developed linear-after-the-exponential rolling …

th, cn, jp, ru (code pays fourni par la source)

Allosteric stem-loop multicomponent DNAzyme: A versatile stimuli-responsive switch for nanotweezer operations and multifunctional biosensing

Yaoyi Zhang, Dan Bai, Jiu Pu, Li Zhang et autres

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