Scott A. Wilson

The effects of introduced procedural errors on malaria rapid diagnostic test performance in a laboratory setting

Scott A. Wilson, Yong Ah, Michael Aidoo

BACKGROUND: Rapid Diagnostic Tests (RDTs) are the primary means of malaria diagnosis in sub-Saharan Africa. Outside large health facilities, individuals performing RDTs have little or no formal laboratory training, and performing RDTs is by job aids derived from manufacturers' instructions for use …

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The effects of introduced procedural errors on malaria rapid diagnostic test performance in a laboratory setting

Scott A. Wilson, Yong Ah, Michael Aidoo

Abstract Background Rapid Diagnostic Tests (RDTs) are the primary means of malaria diagnosis in sub-Saharan Africa. Outside large health facilities, individuals performing RDTs have little or no formal laboratory training, and performing RDTs is by job aids derived from manufacturers’ instructions for …

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Additional file 1 of The effects of introduced procedural errors on malaria rapid diagnostic test performance in a laboratory setting

Scott A. Wilson, Yong Ah, Michael Aidoo

Supplementary material 1.

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The effects of introduced procedural errors on malaria rapid diagnostic test performance in a laboratory setting

Scott A. Wilson, Yong Ah, Michael Aidoo

Abstract Background Rapid Diagnostic Tests (RDTs) are the primary means of malaria diagnosis in sub-Saharan Africa. Outside large health facilities, individuals performing RDTs have little or no formal laboratory training, and performing RDTs is by job aids derived from manufacturers’ instructions for …

us (code pays fourni par la source)

Additional file 1 of The effects of introduced procedural errors on malaria rapid diagnostic test performance in a laboratory setting

Scott A. Wilson, Yong Ah, Michael Aidoo

Supplementary material 1.

us (code pays fourni par la source)

Purification of native histidine-rich protein 2 (nHRP2) from Plasmodium falciparum culture supernatant, infected RBCs, and parasite lysate

Balwan Singh, Jessica N. McCaffery, Amy Kong, Yong Ah et autres

BACKGROUND: Despite the widespread use of histidine-rich protein 2 (HRP2)-based rapid diagnostic tests (RDTs), purified native HRP2 antigen is not standardly used in research applications or assessment of RDTs used in the field. METHODS: This report describes the purification of native HRP2 …

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HRP2 and HRP3 cross-reactivity and implications for HRP2-based RDT use in regions with Plasmodium falciparum hrp2 gene deletions

Amy Kong, Scott A. Wilson, Yong Ah, Douglas Nace et autres

BACKGROUND: The Plasmodium falciparum antigen histidine rich protein 2 (HRP2) is a preferred target for malaria rapid diagnostic tests (RDTs) because of its abundant production by the parasite and thermal stability. As a result, a majority of RDTs procured globally target this …

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Impact of Plasmodium falciparum gene deletions on malaria rapid diagnostic test performance

Michelle L. Gatton, Jeff Glenn, Scott A. Wilson, Yong Ah et autres

BACKGROUND: Malaria rapid diagnostic tests (RDTs) have greatly improved access to diagnosis in endemic countries. Most RDTs detect Plasmodium falciparum histidine-rich protein 2 (HRP2), but their sensitivity is seriously threatened by the emergence of pfhrp2-deleted parasites. RDTs detecting P. falciparum or pan-lactate …

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Shear-Thinning and Thermo-Reversible Nanoengineered Inks for 3D Bioprinting

Scott A. Wilson, Lauren Cross, Charles W. Peak, Akhilesh K. Gaharwar

Three-dimensional (3D) printing is an emerging approach for rapid fabrication of complex tissue structures using cell-loaded bioinks. However, 3D bioprinting has hit a bottleneck in progress because of the lack of suitable bioinks that are printable, have high shape fidelity, and are …

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Designing Advanced Bioinks for 3D Printing Complex Tissue Structures

Scott A. Wilson

Three-dimensional (3D) printing is an emerging approach for rapid fabrication of complex tissue structures using cell-loaded bioinks. However, 3D bioprinting has hit a bottleneck in progress due to the lack of suitable bioinks that are printable, have high shape fidelity, and are …

SPARSELY SAMPLED WIDEBAND RADAR HOLOGRAPHIC IMAGING FOR DETECTION OF CONCEALED OBJECTS

Ram M. Narayanan, Scott A. Wilson, Muralidhar Rangaswamy

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Low-Cost 3D Printers Enable High-Quality and Automated Sample Preparation and Molecular Detection

Kamfai Chan, Mauricio D. Coen, Justin P. Hardick, Charlotte Ann Gaydos et autres

Most molecular diagnostic assays require upfront sample preparation steps to isolate the target's nucleic acids, followed by its amplification and detection using various nucleic acid amplification techniques. Because molecular diagnostic methods are generally rather difficult to perform manually without highly trained users, …

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