Accès ouvert
2026
preprint
OpenAlex
Helena Schulz-Mirbach, Vittorio Rainaldi, Nitin Bohra, Katsutaka Suzuki et autres
Abstract De novo metabolic pathways open possibilities for sustainable biotransformations in microbes. However, the in vivo -implementation of such new-to-nature pathways is highly challenging and heavily relies on adaptive laboratory evolution (ALE) of the host’s native metabolic network. Here, we assess how …
de, nl, us, ca
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
René Inckemann, Tanguy Chotel, Michael Burgis, Cedric K. Brinkmann et autres
Chloroplast synthetic biology holds promise for advancing photosynthetic organisms through improving the function of plastids. However, chloroplast engineering efforts face limitations due to the scarcity of genetic tools and the low throughput of plant-based systems. To address these challenges, we here established …
de, gb
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Hai He, Paul A. Gómez-Coronado, Jan Zarzycki, Sebastian Barthel et autres
Abstract Promiscuous enzymes often serve as the starting point for the evolution of novel functions. Yet, the extent to which the promiscuity of an individual enzyme can be harnessed several times independently for different purposes during evolution is poorly reported. Here, we …
de, us
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
René Inckemann, Tanguy Chotel, Cedric K. Brinkmann, Michael Burgis et autres
Abstract Chloroplast synthetic biology holds promise for developing improved crops through improving the function of plastids. However, chloroplast engineering efforts face limitations due to the scarcity of genetic tools and the low throughput of plant-based systems. To address these challenges, we here …
de, us, gb
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Shanshan Luo, Christoph Diehl, Hai He, YoungJun Bae et autres
Abstract Synthetic biology offers the opportunity to build solutions for improved capture and conversion of carbon dioxide (CO2) that outcompete those evolved by nature. Here we demonstrate the design and construction of a new-to-nature CO2-fixation pathway, the reductive tricarboxylic acid branch/4-hydroxybutyryl-CoA/ethylmalonyl-CoA/acetyl-CoA (THETA) …
de, us, nl
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Shanshan Luo, David Adam, Simone Giaveri, Sebastian Barthel et autres
Electricity is paramount to the technical world and plays an increasingly important role as a future energy carrier. Yet, it is not widely used to directly power biological systems. Here, we designed a new-to-nature electrobiological module, the acid/aldehyde ATP cycle (AAA cycle), …
de, us
(code pays fourni par la source)
Accès ouvert
2021
article
OpenAlex
Vivian Meyer, Melanie Klose, Alexander Lerchl
BACKGROUND: Spermatogenesis is an elaborately organized and tightly regulated differentiation process. The spermatogenesis duration is stable within a certain species but highly variable between species of the same family. OBJECTIVES: In this study, the spermatogenesis duration of the Roborovski hamster was measured …
de
(code pays fourni par la source)
Accès ouvert
2021
article
OpenAlex
Marieke Scheffen, Daniel G. Marchal, Thomas Beneyton, Sandra K. Schuller et autres
Abstract The capture of CO 2 by carboxylases is key to sustainable biocatalysis and a carbon-neutral bio-economy, yet currently limited to few naturally existing enzymes. Here, we developed glycolyl-CoA carboxylase (GCC), a new-to-nature enzyme, by combining rational design, high-throughput microfluidics and microplate …
de, fr
(code pays fourni par la source)
Accès ouvert
2020
article
OpenAlex
Alexander Lerchl, Melanie Klose, Karen Drees
The widespread use of mobile phones and Wi-Fi-based communication devices makes exposure to radiofrequency electromagnetic fields (RF-EMF) unavoidable. Previous experiments have revealed the tumor-promoting effects of non-ionizing RF-EMF in adult carcinogen-treated mice in utero. To extend these investigations, we tested whether these …
de
(code pays fourni par la source)
Accès ouvert
2020
article
OpenAlex
Ralf Conrad, Melanie Klose, Alex Enrich‐Prast
Lake sediments in Amazonia are a significant source of CH 4 , a potential greenhouse gas. Previous studies of sediments using 13 C analysis found that the contribution of hydrogenotrophic versus acetoclastic methanogenesis to CH 4 production was relatively high. Here, we …
de, se, br
(code pays fourni par la source)
Accès ouvert
2019
preprint
OpenAlex
Ralf Conrad, Melanie Klose, Alex Enrich‐Prast
Abstract. Lake sediments in Amazonia are a significant source of CH4, a potential greenhouse gas. Previous studies of sediments using 13C analysis found that the contribution of hydrogenotrophic versus aceticlastic methanogenesis to CH4 production was relatively high. Here, we determined the methanogenic …
de, se, br
(code pays fourni par la source)
Accès ouvert
2019
article
OpenAlex
Pengfei Liu, Melanie Klose, Ralf Conrad
Temperature is an important factor regulating the production of the greenhouse gas CH4. Structure and function of the soil methanogenic microbial communities are often drastically different upon incubation at 45°C versus 25 or 35°C, but are also different in different soils. Therefore, …
de
(code pays fourni par la source)