Chuang Li

The Microtubule‐Associated Protein CsTON2 Interacts With CsTRM5 and CsSUN to Regulate Fruit Shape Development in Cucumber

M. F. Li, Xiaoli Li, Yuting He, Chuang Li et autres

Fruit shape is an important external quality trait that directly determines the market value. Modification of fruit shape has emerged as a key focus in crop improvement, but the regulatory network of fruit shape specifications remains largely unknown. Here, we identified a …

cn (code pays fourni par la source)

Ultrahigh-Throughput Förster Resonance Energy Transfer-Activated Droplet Sorting for Terminator Polymerase Engineering

Hui Zhang, Qingqing Xie, Haoran Xu, Wei Li et autres

Abstract Droplet microfluidic screening systems enable high-throughput, labor-saving enzyme directed evolution by employing fluorescence, absorbance, and Raman-activated sorting strategies for library screening. Förster resonance energy transfer (FRET) – a nanoscale technique for monitoring intramolecular/intermolecular conformational changes – is yet to be integrated …

cn, Botswana (code pays fourni par la source)

ARF3-mediated auxin signaling is essential for sex determination in cucumber

Lijie Han, Min Li, Chuang Li, Bosi Zhao et autres

Sex determination underpins genesis of male and female flowers with particularly important implications in plant breeding. Auxin and ethylene regulate femaleness in cucurbits. In this work, we identified an auxin response factor 3 ( CsARF3 ) that plays an essential role in …

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GLM-4.5: Agentic, Reasoning, and Coding (ARC) Foundation Models

Team, Aohan Zeng, Xin Lü, Qinkai Zheng et autres

We present GLM-4.5, an open-source Mixture-of-Experts (MoE) large language model with 355B total parameters and 32B activated parameters, featuring a hybrid reasoning method that supports both thinking and direct response modes. Through multi-stage training on 23T tokens and comprehensive post-training with expert …

The CsphyB–CsPIF4–CsBRC1 module regulates ABA biosynthesis and axillary bud outgrowth in cucumber

Ye Liu, Zhihan Liu, Chuang Li, Min Li et autres

Shoot branching is an important crop agronomic trait that directly affects plant architecture and crop productivity. Although phytochrome B (phyB), BRANCHED1 (BRC1), and abscisic acid (ABA) mediate axillary bud outgrowth, it is unknown if there is any integrating factor among them in …

cn, us (code pays fourni par la source)

Integration of transcriptome and metabolome reveals key regulatory defense pathways associated with high temperature stress in cucumber (Cucumis sativus L.)

Yong Yuan, Xiao Ma, Chuang Li, Xitong Zhong et autres

High temperature stress seriously affects the quality and yield of vegetable crops, especially cucumber ( Cucumis sativus L.). However, the metabolic dynamics and gene regulatory network of cucumber in response to high temperature stress remain poorly studied. In this study, we identified …

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Single nucleotide polymorphisms in SEPALLATA 2 underlie fruit length variation in cucurbits

Weiyuan Song, Yang Xie, Bin Liu, Yuxiang Huang et autres

Complete disruption of critical genes is generally accompanied by severe growth and developmental defects, which dramatically hinder its utilization in crop breeding. Identifying subtle changes, such as single-nucleotide polymorphisms (SNPs), in critical genes that specifically modulate a favorable trait is a prerequisite …

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Heterotrimeric Gα-subunit regulates flower and fruit development in CLAVATA signaling pathway in cucumber

Lijie Han, Yafei Huang, Chuang Li, Di Tian et autres

Abstract Flowers and fruits are the reproductive organs in plants and play essential roles in natural beauty and the human diet. CLAVATA (CLV) signaling has been well characterized as regulating floral organ development by modulating shoot apical meristem (SAM) size; however, the …

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The AGAMOUS-LIKE 16–GENERAL REGULATORY FACTOR 1 module regulates axillary bud outgrowth via catabolism of abscisic acid in cucumber

Jiacai Chen, Liu Liu, Guanghui Wang, Guangxin Chen et autres

Lateral branches are important components of shoot architecture and directly affect crop yield and production cost. Although sporadic studies have implicated abscisic acid (ABA) biosynthesis in axillary bud outgrowth, the function of ABA catabolism and its upstream regulators in shoot branching remain …

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