J. Patrick Murphy

Serine Starvation Modulates mRNA Splicing through Translational Inhibition of SRSF Proteins

Philippa A. Burns, Negar Tabatabaei, Laura M. Selfors, Huiping Zhao et autres

Abstract Serine is a critical nutrient for cancer cell proliferation due to its numerous downstream biosynthetic functions. Given the ongoing clinical efforts to utilize dietary serine starvation as a potential therapy for serine auxotrophic tumors, it is important to understand how cancer …

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Promoter hijacking by primate LINC00473 disrupts an ancestral CREB-PDE10A feedback loop

Shelby J. Squires, Brandon Smith, Mukhayyo Sultonova, V. Shruthi Bandi et autres

ABSTRACT Thousands of human long noncoding RNAs (lncRNAs) evolved in primates, although much remains unknown about how these lncRNAs shaped human gene regulatory networks. The primate-specific lncRNA gene LINC00473 has a CREB-inducible promoter that is conserved beyond primates and the gene is …

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Self-Assembled Peptide Nanoparticle-Mediated Macrophage Polarization Enhances Anticancer Efficacy of Chemotherapeutics in Triple-Negative Breast Cancer

Adnan Murad Bhayo, Nauman Nazeer, Mukhayyo Sultonova, João A. Paulo et autres

Peptide-based self-assembled materials are being investigated as a biodegradable option with various biomedical applications such as drug-delivery carriers and as biosensors. In this study, we report the mechanism of action of a synthetic β-sheet and turn rich macrocyclic host defense peptide that …

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Metabolite Functions Revealed by Mass Spectrometry-Based Target Engagement Proteomics Approaches

Nisha A. Owens, Tayah B. Sommer, Brenna G. Ing, Mukhayyo Sultonova et autres

In addition to their roles in energetics and biosynthesis, endogenous metabolites have functional roles performed in part through protein interactions that result in allosteric regulation, transcriptional regulation, and post-translational modifications. Novel bioactive roles for metabolites continue to emerge in cancer progression, immune …

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Editorial Expression of Concern: The NAD+ salvage pathway modulates cancer cell viability via p73

Tanveer Sharif, Dae‐Gyun Ahn, Rong‐Zong Liu, Eric S. Pringle et autres

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Carrier-Guided Proteome Analysis in a High Protein Background: An Improved Approach to Host Cell Protein Identification

Divyanshi Karmani, Niloofar Seifihesar, Mukhayyo Sultonova, Beau Blackmore et autres

Many shotgun proteomics experiments are negatively influenced by highly abundant proteins, such as those measuring residual host cell proteins (HCP) amidst highly abundant recombinant biotherapeutic or plasma proteins amidst albumin and immunoglobulins. While western blotting and ELISAs can reveal the presence of …

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Bacterial growth‐mediated systems remodelling of Nicotiana benthamiana defines unique signatures of target protein production in molecular pharming

Nicholas Prudhomme, Rebecca Pastora, Sarah Thomson, Edison Zheng et autres

The need for therapeutics to treat a plethora of medical conditions and diseases is on the rise and the demand for alternative approaches to mammalian-based production systems is increasing. Plant-based strategies provide a safe and effective alternative to produce biological drugs but …

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Rapid recruitment and IFN-I–mediated activation of monocytes dictate focal radiotherapy efficacy

Sirimuvva Tadepalli, Derek R. Clements, Sanjana Saravanan, Rebeca Arroyo Hornero et autres

The recruitment of monocytes and their differentiation into immunosuppressive cells is associated with the low efficacy of preclinical nonconformal radiotherapy (RT) for tumors. However, nonconformal RT (non-CRT) does not mimic clinical practice, and little is known about the role of monocytes after …

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Data from Inhibition of Pyruvate Dehydrogenase Kinase Enhances the Antitumor Efficacy of Oncolytic Reovirus

Barry E. Kennedy, J. Patrick Murphy, Derek R. Clements, Prathyusha Konda et autres

Abstract Oncolytic viruses (OV) such as reovirus preferentially infect and kill cancer cells. Thus, the mechanisms that dictate the susceptibility of cancer cells to OV-induced cytotoxicity hold the key to their success in clinics. Here, we investigated whether cancer cell metabolism defines …

Supplementary Data from Inhibition of Pyruvate Dehydrogenase Kinase Enhances the Antitumor Efficacy of Oncolytic Reovirus

Barry E. Kennedy, J. Patrick Murphy, Derek R. Clements, Prathyusha Konda et autres

Raw values for all in vitro and in vivo metabolomic experiments are presented in supplemental tables 1, 7, and 8. Supplemental tables 2, 3, 4, and 5 contain the correlation values and enrichment analysis for all experiments. Supplemental table 6 contains raw …

Supplementary Data from Inhibition of Pyruvate Dehydrogenase Kinase Enhances the Antitumor Efficacy of Oncolytic Reovirus

Barry E. Kennedy, J. Patrick Murphy, Derek R. Clements, Prathyusha Konda et autres

Supplemental figures 1-6 contain metabolomic, proteomic, extracellular flux analysis, cell death, and in vivo data supporting our findings that pyruvate dehydrogenase activity influences oncolytic viral therapy.

Supplementary Data from Inhibition of Pyruvate Dehydrogenase Kinase Enhances the Antitumor Efficacy of Oncolytic Reovirus

Barry E. Kennedy, J. Patrick Murphy, Derek R. Clements, Prathyusha Konda et autres

Supplementary Figure Legends