Atsushi Fukuda

Multimodal AI for Bacterial vs Viral Upper Respiratory Tract Infection Multicentre Development and Internal Validation

Sho Okiyama, Yuji Ariyasu, Hiroshi Yoshihara, Atsushi Fukuda et autres

jp, us (code pays fourni par la source)

An XCI-Preserved Mosaic Model of Rett Syndrome Reveals Context-Dependent Neurodevelopment and Stage-Specific Responses to MECP2 Restoration

Kosei Hayakawa, Nami Motosugi, Atsushi Fukuda, See PDF

jp (code pays fourni par la source)

Epigenetic Reprogramming and Zygotic Genome Activation in Human Preimplantation Development: Mechanisms, Models, and Translational Prospects

Atsushi Fukuda

Purpose: Early human embryogenesis unfolds through a tightly coupled sequence of events-clearance of maternal transcripts, remodeling of parental chromatin, zygotic genome activation (ZGA), lineage segregation, implantation, and post-implantation patterning-accompanied by epigenetic reprogramming, including X-chromosome dosage compensation around the time of implantation. This …

jp (code pays fourni par la source)

Upregulation of the lncRNA XACT sustains pluripotency, blocks lineage specification, and drives germ cell tumor-like transcriptional programs in human pluripotent stem cells

Kenji Izumi, Nami Motosugi, Akiko Sugiyama, Natsumi Kurosaki et autres

Summary Long noncoding RNAs (lncRNAs) represent a vast class of regulatory transcripts and are spatiotemporally controlled, yet only a few have been functionally implicated in human development. Here, we identify the X-linked lncRNA XACT , abundantly but transiently expressed during early human …

jp (code pays fourni par la source)

Highly efficient XIST reactivation in female hPSC by transient dual inhibition of TP53 and DNA methylation during Cas9 mediated genome editing

Nami Motosugi, K. Hasegawa, Natsumi Kurosaki, Kenji Izumi et autres

The irreversible erosion of X-chromosome inactivation (XCI) due to repression of the long non-coding RNA XIST presents a major challenge for disease modeling and raises safety concerns for the clinical application of female human pluripotent stem cells (hPSCs) due to the aberrant …

jp (code pays fourni par la source)

From Brainwaves to Brain Scans: A Robust Neural Network for EEG-to-fMRI Synthesis

Atsushi Fukuda, Quan Huu

While functional magnetic resonance imaging (fMRI) offers valuable insights into brain activity, it is limited by high operational costs and significant infrastructural demands. In contrast, electroencephalography (EEG) provides millisecond-level precision in capturing electrical activity but lacks the spatial fidelity necessary for precise …

From Brainwaves to Brain Scans: A Robust Neural Network for EEG-to-fMRI Synthesis

Atsushi Fukuda, Quan Huu Cap

While functional magnetic resonance imaging (fMRI) offers valuable insights into brain activity, it is limited by high operational costs and significant infrastructural demands. In contrast, electroencephalography (EEG) provides millisecond-level precision in capturing electrical activity but lacks the spatial fidelity necessary for precise …

Highly efficient XIST reactivation in female hPSC by transient dual inhibition of TP53 and DNA methylation during Cas9 mediated genome editing

Nami Motosugi, K. Hasegawa, Natsumi Kurosaki, Kenji Izumi et autres

Summary Erosion of X-chromosome inactivation (XCI) complicates disease and developmental modeling in female human pluripotent stem cells (hPSCs). Previous studies demonstrated that Cas9-mediated editing of the XIST promoter via non-homologous end joining (NHEJ) or homology-directed repair (HDR) with a selection cassette upstream …

jp (code pays fourni par la source)

Effect of PCDH19 missense mutations on cell-to-cell proximity and neuronal development under heterotypic conditions

Nami Motosugi, Akiko Sugiyama, Asako Otomo, Yuka Sakata et autres

The mutation of the X-linked protocadherin (PCDH) 19 gene in heterozygous females causes epilepsy. However, because of the erosion of X-chromosome inactivation (XCI) in female human pluripotent stem cells, precise disease modeling often leads to failure. In this study, using a mathematical …

jp (code pays fourni par la source)

Nanosheet coating improves stability of human pluripotent stem cell culture on glass substrates

Yuka Sakata, Hong Zhang, Akiko Sugiyama, Nami Motosugi et autres

High-resolution imaging analysis using various types of cells is an essential tool for dissecting cell functions. Generally, obtaining such images requires the cells to be cultured on glass substrates; however, it often results in the unstable status of cells. In this study, …

jp, cn (code pays fourni par la source)

De-erosion of X chromosome dosage compensation by the editing of XIST regulatory regions restores the differentiation potential in hPSCs

Nami Motosugi, Akiko Sugiyama, Chisa Okada, Asako Otomo et autres

Human pluripotent stem cells (hPSCs) regularly and irreversibly show the erosion of X chromosome inactivation (XCI) by long non-coding RNA (lncRNA) XIST silencing, causing challenges in various applications of female hPSCs. Here, we report reliable methods to reactivate XIST with monoallelic expression …

jp (code pays fourni par la source)

Genetic Characterization of Staphylococcus aureus, Staphylococcus argenteus, and Coagulase-Negative Staphylococci Colonizing Oral Cavity and Hand of Healthy Adults in Northern Japan

Mina Hirose, Meiji Soe Aung, Yusuke Fujita, Taisei Kato et autres

The spread of methicillin resistance and virulence among staphylococci in the community poses a public health concern. In this study, we investigated the prevalence of Staphylococcus species colonizing the oral cavity and hand (skin) of healthy university students and their phenotypic and …

jp (code pays fourni par la source)